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BOD Bottle BOD test bottles at the laboratory of a wastewater treatment plant. BOD Bottle or an incubation bottle is a main apparatus used for the Biological Oxygen Demand (BOD) test. During the five-day BOD or BOD5 test process, the BOD bottle is used for incubating diluted samples under the 20 °C or 68 °F of temperature.
BOD test bottles at the laboratory of a wastewater treatment plant. Biochemical oxygen demand (also known as BOD or biological oxygen demand) is an analytical parameter representing the amount of dissolved oxygen (DO) consumed by aerobic bacteria growing on the organic material present in a water sample at a specific temperature over a specific time period.
The difference in DO and the dilution factor are used to calculated BOD 5. The resulting number (usually reported in parts per million or milligrams per liter) is useful in determining the relative organic strength of sewage or other polluted waters. The BOD 5 test is an example of analysis that determines classes of materials in a sample.
Both the BOD and COD tests are a measure of the relative oxygen-depletion effect of a waste contaminant. Both have been widely adopted as a measure of pollution effect. The BOD test measures the oxygen demand of biodegradable pollutants whereas the COD test measures the oxygen demand of oxidizable pollutants.
A standard operating procedure (SOP) is a set of step-by-step instructions compiled by an organization to help workers carry out routine operations. [1] SOPs aim to achieve efficiency, quality output, and uniformity of performance, while reducing miscommunication and failure to comply with industry regulations .
Inside a shaking water bath A water bath operating at 72°C. A water bath is laboratory equipment made from a container filled with heated water. It is used to incubate samples in water at a constant temperature over a long period of time.
The laboratory procedure involves making serial dilutions of the sample (1:10, 1:100, 1:1000, etc.) in sterile water and cultivating these on nutrient agar in a dish that is sealed and incubated. Typical media include plate count agar for a general count or MacConkey agar to count Gram-negative bacteria such as E. coli .
Incubators are essential for much experimental work in cell biology, microbiology and molecular biology and are used to culture both bacterial and eukaryotic cells. An incubator is made up of a chamber with a regulated temperature. Some incubators also regulate humidity, gas composition, or ventilation within that chamber.
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