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A full-wave plate introduces a phase difference of exactly one wavelength between the two polarization directions, for one wavelength of light. In optical mineralogy, it is common to use a full-wave plate designed for green light (a wavelength near 540 nm). Linearly polarized white light which passes through the plate becomes elliptically ...
Polarized light microscopy can mean any of a number of optical microscopy techniques involving polarized light. Simple techniques include illumination of the sample with polarized light. Directly transmitted light can, optionally, be blocked with a polariser oriented at 90 degrees to the illumination.
Pulsed DC is commonly produced from AC (alternating current) by a half-wave rectifier or a full-wave rectifier. Full wave rectified ac is more commonly known as Rectified AC. PDC has some characteristics of both alternating current (AC) and direct current (DC) waveforms. The voltage of a DC wave is roughly constant, whereas the voltage of an AC ...
In addition to modifications of the microscope's optical system, petrographic microscopes allow for the insertion of specially-cut oriented filters of biaxial minerals (the quartz wedge, quarter-wave mica plate and half-wave mica plate), into the optical train between the polarizers to identify positive and negative birefringence, and in ...
A two-photon microscope is also a laser-scanning microscope, but instead of UV, blue or green laser light, a pulsed infrared laser is used for excitation. Only in the tiny focus of the laser is the intensity high enough to generate fluorescence by two-photon excitation , which means that no out-of-focus fluorescence is generated, and no pinhole ...
Diagram illustrating the light path through a dark-field microscope. The steps are illustrated in the figure where an inverted microscope is used. Light enters the microscope for illumination of the sample. A specially sized disc, the patch stop (see figure), blocks some light from the light source, leaving an outer ring of illumination. A wide ...
Fluorescence and confocal microscopes operating principle. Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. [1]
Memorial in Jena, Germany to Ernst Karl Abbe, who approximated the diffraction limit of a microscope as = , where d is the resolvable feature size, λ is the wavelength of light, n is the index of refraction of the medium being imaged in, and θ (depicted as α in the inscription) is the half-angle subtended by the optical objective lens (representing the numerical aperture).