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Resolution (chromatography) Example chromatogram showing signal as a function of retention time. In chromatography, resolution is a measure of the separation of two peaks of different retention time t in a chromatogram. [1][2][3][4]
The fundamental resolution equation is used in chromatography to help relate adjustable chromatographic parameters to resolution, and is as follows: R s = [N 1/2 /4] [ (α-1)/α] [k 2 '/ (1+k 2 ')], where. N = Number of theoretical plates. α = Selectivity Term = k 2 '/k 1 '. The [N 1/2 /4] term is the column factor, the [ (α-1)/α] term is ...
In order to improve separation efficiency and peak resolution, ultra performance liquid chromatography (UHPLC) can be used instead of HPLC. This LC variant uses columns packed with smaller silica particles (~1.7 μm diameter) and requires higher operating pressures in the range of 310000 to 775000 torr (6000 to 15000 psi, 400 to 1034 bar).
The greater the separation factor value is over 1.0, the better the separation, until about 2.0 beyond which an HPLC method is probably not needed for separation. Resolution equations relate the three factors such that high efficiency and separation factors improve the resolution of component peaks in an HPLC separation.
IUPAC definition. The IUPAC definition for resolution in mass spectrometry is. Where a larger resolution indicates a better separation of peaks. [1][2] This definition is used in a number of mass spectrometry texts. [3][4][5][6][7][8][9][10][11] This use is also implied by the term "high-resolution mass spectrometry." [12]
A chemist in the 1950s using column chromatography. The Erlenmeyer receptacles are on the floor. Column chromatography in chemistry is a chromatography method used to isolate a single chemical compound from a mixture. Chromatography is able to separate substances based on differential adsorption of compounds to the adsorbent; compounds move ...
The response factor can be expressed on a molar, volume or mass [1] basis. Where the true amount of sample and standard are equal: = where A is the signal (e.g. peak area) and the subscript i indicates the sample and the subscript st indicates the standard. [2]
The van Deemter equation is a hyperbolic function that predicts that there is an optimum velocity at which there will be the minimum variance per unit column length and, thence, a maximum efficiency. The van Deemter equation was the result of the first application of rate theory to the chromatography elution process.
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