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Capillary electrophoresis (CE) has become an important, cost-effective approach to do DNA sequencing that provides high throughput and high accuracy sequencing information. Woolley and Mathies used a CE chip to sequence DNA fragments with 97% accuracy and a speed of 150 bases in 540 seconds. [22]
Microfluidic Sanger sequencing is a lab-on-a-chip application for DNA sequencing, in which the Sanger sequencing steps (thermal cycling, sample purification, and capillary electrophoresis) are integrated on a wafer-scale chip using nanoliter-scale sample volumes. This technology generates long and accurate sequence reads, while obviating many ...
DNA sequencing is the process of determining the nucleic acid sequence – the order of ... such as fluorescent labelling, capillary electrophoresis, and general ...
The data is plotted with time, shown via base pairs (bps), on the x-axis and fluorescence intensity on the y-axis. Such plots are often achieved using an instrument such as an automated DNA sequencer paired with capillary electrophoresis (CE). Such electropherograms may be used to determine DNA sequence genotypes, or genotypes that are based on ...
The next major advance was the release in 1995 of the AB310 which utilized a linear polymer in a capillary in place of the slab gel for DNA strand separation by electrophoresis. These techniques formed the base for the completion of the human genome project in 2001. [4]
If the DNA fragment to be analyzed is produced by polymerase chain reaction (PCR), it is straightforward to couple a fluorescent molecule such as carboxyfluorescein (FAM) to the primers. This way, the fragments produced by DNaseI digestion will contain FAM, and will be detectable by the capillary electrophoresis machine.
Sequencing methods have evolved from relatively laborious gel-based procedures to modern automated protocols based on dye labelling and detection in capillary electrophoresis that permit rapid large-scale sequencing of genomes and transcriptomes. [1]
DNA sequencing is the process of determining the nucleotide order of a given DNA fragment. So far, most DNA sequencing has been performed using the chain termination method developed by Frederick Sanger. This technique uses sequence-specific termination of a DNA synthesis reaction using modified nucleotide substrates.
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