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Bacterial growth curve\Kinetic Curve. In autecological studies, the growth of bacteria (or other microorganisms, as protozoa, microalgae or yeasts) in batch culture can be modeled with four different phases: lag phase (A), log phase or exponential phase (B), stationary phase (C), and death phase (D). [3]
Figure 1: A bi-phasic bacterial growth curve.. A growth curve is an empirical model of the evolution of a quantity over time. Growth curves are widely used in biology for quantities such as population size or biomass (in population ecology and demography, for population growth analysis), individual body height or biomass (in physiology, for growth analysis of individuals).
[1] [2] They may either kill or inhibit the growth of bacteria. A limited number of antibiotics also possess antiprotozoal activity. [3] [4] Antibiotics are not effective against viruses such as the ones which cause the common cold or influenza. [5] Drugs which inhibit growth of viruses are termed antiviral drugs or antivirals.
Narrow-spectrum antibiotics have low propensity to induce bacterial resistance and are less likely to disrupt the microbiome (normal microflora). [3] On the other hand, indiscriminate use of broad-spectrum antibiotics may not only induce the development of bacterial resistance and promote the emergency of multidrug-resistant organisms, but also cause off-target effects due to dysbiosis.
μ is the growth rate of a considered microorganism, μ max is the maximum growth rate of this microorganism, [S] is the concentration of the limiting substrate S for growth, K s is the "half-velocity constant"—the value of [S] when μ/μ max = 0.5. μ max and K s are empirical (experimental) coefficients to the Monod equation. They will ...
Bacteriostatic antibiotics limit the growth of bacteria by interfering with bacterial protein production, DNA replication, or other aspects of bacterial cellular metabolism. They must work together with the immune system to remove the microorganisms from the body.
Antibiotics to which a bacterial isolate is partially susceptible will produce an intermediate size zone of inhibition (disk B). [1] [2] In drug discovery laboratories, the disk diffusion test is used to screen natural product extracts for antibacterial activity. Extracts with antibacterial activity, for example the petroleum ether, chloroform ...
Following the allotted time, the plate is removed and checked for bacterial growth. If the broth became cloudy or a layer of cells formed at the bottom, then bacterial growth has occurred. The results of the broth microdilution method are reported in Minimum Inhibitory Concentration (MIC), or the lowest concentration of antibiotics that stopped ...