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A restriction enzyme, restriction endonuclease, REase, ENase or restrictase is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sites. [1] [2] [3] Restriction enzymes are one class of the broader endonuclease group of enzymes.
Some restriction enzymes cut DNA at a restriction site in a manner which leaves no overhang, called a blunt end. [2] Blunt ends are much less likely to be ligated by a DNA ligase because the blunt end doesn't have the overhanging base pair that the enzyme can recognize and match with a complementary pair. [3]
The restriction modification system (RM system) is found in bacteria and archaea, and provides a defense against foreign DNA, such as that borne by bacteriophages. Bacteria have restriction enzymes , also called restriction endonucleases , which cleave double-stranded DNA at specific points into fragments, which are then degraded further by ...
A deed restriction is a legal stipulation written into the deed of a property that dictates certain conditions or limitations on use. These restrictions can range from architectural guidelines to ...
In mathematics, the restriction of a function is a new function, denoted | or , obtained by choosing a smaller domain for the original function . The function f {\displaystyle f} is then said to extend f | A . {\displaystyle f\vert _{A}.}
A restriction fragment is a DNA fragment resulting from the cutting of a DNA strand by a restriction enzyme (restriction endonucleases), a process called restriction. [1] Each restriction enzyme is highly specific, recognising a particular short DNA sequence, or restriction site, and cutting both DNA strands at specific points within this site.
Restriction enzyme, a type of enzyme that cleaves genetic material; Mathematics and logic. Restriction, a term in medieval supposition theory;
Restriction digest is most commonly used as part of the process of the molecular cloning of DNA fragment into a vector (such as a cloning vector or an expression vector).The vector typically contains a multiple cloning site where many restriction site may be found, and a foreign piece of DNA may be inserted into the vector by first cutting the restriction sites in the vector as well the DNA ...