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Hydrophilic interaction chromatography (or hydrophilic interaction liquid chromatography, HILIC) [1] is a variant of normal phase liquid chromatography that partly overlaps with other chromatographic applications such as ion chromatography and reversed phase liquid chromatography. HILIC uses hydrophilic stationary phases with reversed-phase ...
Just as in hydrophilic interaction chromatography (HILIC; a sub-technique within HPLC), this method separates analytes based on differences in their polarity. HILIC most often uses a bonded polar stationary phase and a mobile phase made primarily of acetonitrile with water as the strong component. Partition HPLC has been used historically on ...
[13] Higher loading capacity, [14] [15] [16] for example, loading capacity of ACE/ hydrophilic interaction chromatography (HILIC) increased 10-100 times when compared with RPLC, [15] which offered a new selection and idea for developing semi-preparative and preparative chromatography.
N-Acetyltaurine can be quantified by a combination of high performance liquid chromatography and mass spectrometry (MS/MS). Due to the high hydrophilicity of N-acetyltaurine, the hydrophilic interaction chromatography (HILIC) is the method of choice in order to separate the analyte from the matrix components. [3] [4]
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This property distinguishes it from a pure HILIC (hydrophilic interaction chromatography) columns where separation by polar differences is obtained through partitioning into a water-rich layer on the surface, or a pure RP stationary phase on which separation by nonpolar differences in solutes is obtained with very limited secondary mechanisms ...
Just about four years ago, at the height of COVID, we lost photographer and activist Corky Lee. His work is the subject of a recent book, "Corky Lee's Asian America: Fifty Years of Photographic ...
Similar recommendations have been issued by other authorities, like the Japanese Ministry of Health, Labor and Welfare, [77] both based on liquid chromatography – mass spectrometry detection after hydrophilic interaction liquid chromatography separation. [78]