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A 0.5 McFarland standard is prepared by mixing 0.05 mL of 1.175% barium chloride dihydrate (BaCl 2 •2H 2 O), with 9.95 mL of 1% sulfuric acid (H 2 SO 4). [ 1 ] Now there are McFarland standards prepared from suspensions of latex particles, which lengthens the shelf life and stability of the suspensions.
Granada agar is used for the primary isolation, identification and screening of β-hemolytic GBS from clinical specimens. [2] [4] This culture medium is selective for GBS, nevertheless other microorganisms (such as enterococci and yeasts), resistant to the selective agents used, can develop as colorless or white colonies. [2]
Hardy Diagnostics is an American company that manufactures and sells bacteriological culture media, reagents, automated microscope slide staining machines, and rapid identification kits for microbiological testing in clinical, research, and industrial laboratories. The company's culture media is useful in the detection of bacterial pathogens ...
The following known results can be used to validate the proper composition of the medium: [1] Escherichia coli – ferments mannite, motile; Klebsiella sp. – ferments mannite, non-motile; Pseudomonas aeruginosa – does not ferment mannite, motile
Buffered charcoal yeast extract (BCYE) agar is a selective growth medium used to culture or grow certain types of bacteria, particularly the Gram-negative species Legionella pneumophila. [1] It has also been used for the laboratory diagnosis of Acanthamoeba keratitis , [ 2 ] Francisella and Nocardia spp .
Blood agar plates (BAPs) contain mammalian blood (usually sheep or horse), typically at a 5–10% concentration. BAPs are enriched, and differential media is used to isolate fastidious organisms and detect hemolytic activity. β-Hemolytic activity will show lysis and complete digestion of red blood cell contents surrounding a colony.
An agar plate – an example of a bacterial growth medium*: Specifically, it is a streak plate; the orange lines and dots are formed by bacterial colonies.. A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation [1] or small plants like the moss Physcomitrella patens. [2]
Bordet–Gengou agar is a type of agar plate optimized to isolate Bordetella, containing blood, potato extract, and glycerol, with an antibiotic such as cephalexin or penicillin and sometimes nicotinamide. [1]