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FCCS is an extension of the fluorescence correlation spectroscopy (FCS) method that uses two fluorescent molecules instead of one that emits different colours. The technique measures coincident green and red intensity fluctuations of distinct molecules that correlate if green and red labelled particles move together through a predefined confocal volume. [2]
Dual color fluorescence cross-correlation spectroscopy (FCCS) measures interactions by cross-correlating two or more fluorescent channels (one channel for each reactant), which distinguishes interactions more sensitively than FCS, particularly when the mass change in the reaction is small.
FCS also tells you the size of the formed complexes so you can measure the stoichiometry of binding. A more powerful methods is fluorescence cross-correlation spectroscopy (FCCS) that employs double labeling techniques and cross-correlation resulting in vastly improved signal-to-noise ratios over FCS. Furthermore, the two-photon and three ...
IAES – Ion induced Auger electron spectroscopy; IBA – Ion beam analysis; IBIC – Ion beam induced charge microscopy; ICP-AES – Inductively coupled plasma atomic emission spectroscopy; ICP-MS – Inductively coupled plasma mass spectrometry; Immunofluorescence; ICR – Ion cyclotron resonance; IETS – Inelastic electron tunneling ...
Federation of Culture, Communication and Entertainment (FCCS; French: Fédération de la culture, communication et spectacle), a union of France that is part of the trade union alliance French Confederation of Management – General Confederation of Executives (CFE-CGC) Fluorescence cross-correlation spectroscopy (FCCS)
Fluorescence spectroscopy (also known as fluorimetry or spectrofluorometry) is a type of electromagnetic spectroscopy that analyzes fluorescence from a sample. It involves using a beam of light, usually ultraviolet light , that excites the electrons in molecules of certain compounds and causes them to emit light; typically, but not necessarily ...
Other optical techniques are also used: Fluorescence Correlation and Cross-Correlation Spectroscopy (FCS/FCCS) can be used to gain information of fluorophore mobility in the membrane, Fluorescence Resonance Energy Transfer (FRET) can detect when fluorophores are in close proximity and optical tweezer techniques can give information on membrane ...
In 1978 he was appointed assistant professor in the Department of Physics at UIUC. In 1989 he was promoted to professor. At Illinois, Gratton established the Laboratory for Fluorescence Dynamics (LFD) in 1986 with long-term funding from the NIH, bringing advanced microscopy and spectroscopy techniques to the study of biological systems. [1] [2]