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  2. Phasor approach to fluorescence lifetime and spectral imaging

    en.wikipedia.org/wiki/Phasor_approach_to...

    This is a complex function and drawing the imaginary versus real part of this function for all possible lifetimes will be a semicircle where the zero lifetime is located at (1,0) and the infinite lifetime located at (0,0). By changing the lifetime from zero to infinity the phasor point moves along a semicircle from (1,0) to (0,0).

  3. Phasor - Wikipedia

    en.wikipedia.org/wiki/Phasor

    Phasor notation (also known as angle notation) is a mathematical notation used in electronics engineering and electrical engineering.A vector whose polar coordinates are magnitude and angle is written . [13] can represent either the vector (⁡, ⁡) or the complex number ⁡ + ⁡ =, according to Euler's formula with =, both of which have magnitudes of 1.

  4. Optical transfer function - Wikipedia

    en.wikipedia.org/wiki/Optical_transfer_function

    The three-dimensional point spread functions (a,c) and corresponding modulation transfer functions (b,d) of a wide-field microscope (a,b) and confocal microscope (c,d). In both cases the numerical aperture of the objective is 1.49 and the refractive index of the medium 1.52.

  5. Fluorescence microscope - Wikipedia

    en.wikipedia.org/wiki/Fluorescence_microscope

    A fluorescence microscope is an optical microscope that uses fluorescence instead of, or in addition to, scattering, reflection, and attenuation or absorption, to study the properties of organic or inorganic substances.

  6. Microscopy - Wikipedia

    en.wikipedia.org/wiki/Microscopy

    Scanning electron microscope image of pollen (false colors) Microscopic examination in a biochemical laboratory. Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye (objects that are not within the resolution range of the normal eye). [1]

  7. Köhler illumination - Wikipedia

    en.wikipedia.org/wiki/Köhler_illumination

    Köhler illumination is a method of specimen illumination used for transmitted and reflected light (trans- and epi-illuminated) optical microscopy.Köhler illumination acts to generate an even illumination of the sample and ensures that an image of the illumination source (for example a halogen lamp filament) is not visible in the resulting image.

  8. Point spread function - Wikipedia

    en.wikipedia.org/wiki/Point_spread_function

    By virtue of the linearity property of optical non-coherent imaging systems, i.e., . Image(Object 1 + Object 2) = Image(Object 1) + Image(Object 2). the image of an object in a microscope or telescope as a non-coherent imaging system can be computed by expressing the object-plane field as a weighted sum of 2D impulse functions, and then expressing the image plane field as a weighted sum of the ...

  9. Optical phase space - Wikipedia

    en.wikipedia.org/wiki/Optical_phase_space

    For any such system, a plot of the quadratures against each other, possibly as functions of time, is called a phase diagram. If the quadratures are functions of time then the optical phase diagram can show the evolution of a quantum optical system with time. An optical phase diagram can give insight into the properties and behaviors of the ...