Search results
Results from the WOW.Com Content Network
Microscope image processing is a broad term that covers the use of digital image processing techniques to process, analyze and present images obtained from a microscope. Such processing is now commonplace in a number of diverse fields such as medicine , biological research , cancer research , drug testing , metallurgy , etc.
Images can often be improved – or even transformed – by using graphics software such as Photoshop or the free GIMP and Paint.NET applications. Many other programs also have photo-enhancing tools, including facilities for semi-automatic image enhancement, so that you need only click a button, or choose a thumbnail, to have a positive effect on image quality.
To obtain better image resolution and quality, researchers have used statistical techniques to model the probability where photons may be distributed on the detector. [18] [22] This technique, called the maximum likelihood method, is being further improved by algorithms to improve its performance speed. [22]
Moiré Effect. Structured illumination microscopy (SIM) is a method of super-resolution microscopy which is performed by acquiring multiple images of the same sample under different patterns of illumination, then computationally combining these images to achieve a single reconstruction with up to 2x improvement over the diffraction limited lateral resolution.
The resolution of a microscope is defined as the minimum separation needed between two objects under examination in order for the microscope to discern them as separate objects. This minimum distance is labelled δ. If two objects are separated by a distance shorter than δ, then they will appear as a single object in the microscope.
A higher number of pixels on the same sensor size will not allow the resolution of finer detail. On the other hand, when the pixel spacing is larger than 1 micrometer, the resolution will be limited by the separation between pixels; moreover, aliasing may lead to a further reduction of the image fidelity.
The resolution at that time was limited to 10 µm laterally and 26 µm longitudinally but at a sample size in the millimeter range. The orthogonal-plane fluorescence optical sectioning microscope used a simple cylindrical lens for illumination. Further development and improvement of the selective plane illumination microscope started in 2004. [5]
With no modification to the microscope, i.e. with a simple wide field light microscope, the quality of optical sectioning is governed by the same physics as the depth of field effect in photography. For a high numerical aperture lens, equivalent to a wide aperture, the depth of field is small (shallow focus) and gives good optical sectioning.