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The well position is also standardized, but only for 96- , 384-, and 1536-well plates. These are generally well followed by manufacturers: Well Positions [16] [17] 96-well plates have a 9 mm well-to-well spacing, 384-wells a 4.5 mm spacing, and 1536-wells a 2.25 mm spacing. A notable characteristic is that the well array is symmetrical when the ...
Fractionation at total reflux. The Fenske equation in continuous fractional distillation is an equation used for calculating the minimum number of theoretical plates required for the separation of a binary feed stream by a fractionation column that is being operated at total reflux (i.e., which means that no overhead product distillate is being withdrawn from the column).
Plate readers, also known as microplate readers or microplate photometers, ... (8 by 12 matrix) with a typical reaction volume between 100 and 200 μL per well ...
Orifice plate showing vena contracta. An orifice plate is a thin plate with a hole in it, which is usually placed in a pipe. When a fluid (whether liquid or gaseous) passes through the orifice, its pressure builds up slightly upstream of the orifice [1] but as the fluid is forced to converge to pass through the hole, the velocity increases and the fluid pressure decreases.
An orifice plate meter has a practical turndown ratio of 3:1. A turbine meter has a turndown ratio of 10:1. Rotary positive displacement meters have a turndown ratio of between 10:1 and 80:1, depending on the manufacturer and the application. Diaphragm meters are considered to have a turndown ratio of 80:1.
This assay is presumably very easy to do on a 24-well plate and read with a plate reader; i wouldn't want to count twenty-odd samples on a haemocytometer. The formation of the dye is catalysed by mitochondrial enzymes, so this assay really measures the quantity of those enzymes, right?
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The variance per unit length of the column is taken as the ratio of the column length to the column efficiency in theoretical plates. The van Deemter equation is a hyperbolic function that predicts that there is an optimum velocity at which there will be the minimum variance per unit column length and, thence, a maximum efficiency. The van ...