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For cell seeding the mounted tissue carrier is transferred by a forceps in a 24 well culture plate (Fig. 2). To concentrate cells on top of a tissue carrier culture medium is added to a level so that the selected biomaterial is just wetted. Then an aliquot of cells is transferred by a pipette to the surface of the mounted biomaterial.
The well position is also standardized, but only for 96- , 384-, and 1536-well plates. These are generally well followed by manufacturers: Well Positions [16] [17] 96-well plates have a 9 mm well-to-well spacing, 384-wells a 4.5 mm spacing, and 1536-wells a 2.25 mm spacing. A notable characteristic is that the well array is symmetrical when the ...
In any case, I personally would not be happy plating cells at confluency unless I specifically wanted to monitor cell death of quiescent cells, because the propensity of cells to apoptose varies depending on their stage in the cell cycle. I actually think that this assay is therefore quite misleading.
The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 μL per well. Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per ...
The CFU/plate is read from a plate in the linear range, and then the CFU/g (or CFU/mL) of the original is deduced mathematically, factoring in the amount plated and its dilution factor. A solution of bacteria at an unknown concentration is often serially diluted in order to obtain at least one plate with a countable number of bacteria.
A microtiter plate after an MTT assay. Increasing amounts of cells resulted in increased purple colouring. The MTT assay is a colorimetric assay for assessing cell metabolic activity. [1] [2] NAD(P)H-dependent cellular oxidoreductase enzymes may, under defined conditions, reflect the number of viable cells present.
Spoilers ahead! We've warned you. We mean it. Read no further until you really want some clues or you've completely given up and want the answers ASAP. Get ready for all of today's NYT ...
In microbiology, a culture plate is a low flat-bottomed laboratory container for growing a layer of organisms such as bacteria, molds, and cells on a thin layer of nutrient medium. The most common types are the petri dish [ 1 ] [ 2 ] and multiwell plates .