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It can process single-end and paired-end sequencing data of arbitrary length. cutadapt [25] removes adapter sequences from next-generation sequencing data (Illumina, SOLiD and 454). It is used especially when the read length of the sequencing machine is longer than the sequenced molecule, like the microRNA case.
16S ribosomal RNA (or 16S rRNA) is the RNA component of the 30S subunit of a prokaryotic ribosome . It binds to the Shine-Dalgarno sequence and provides most of the SSU structure. The genes coding for it are referred to as 16S rRNA genes and are used in reconstructing phylogenies, due to the slow rates of evolution of this region of the gene. [2]
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Ribotyping is a molecular technique for bacterial identification and characterization that uses information from rRNA-based phylogenetic analyses. [1] It is a rapid and specific method widely used in clinical diagnostics and analysis of microbial communities in food, water, and beverages.
Rapid amplification of cDNA ends (RACE) is a technique used in molecular biology to obtain the full length sequence of an RNA transcript found within a cell. RACE results in the production of a cDNA copy of the RNA sequence of interest, produced through reverse transcription, followed by PCR amplification of the cDNA copies (see RT-PCR).
Download QR code; Print/export ... Length Accession Reference ... 16S: Escherichia coli: 1,541 nt J01859.1 [1] Archaeal (Prokaryotic) 16S Halobacterium salinarum:
The Cronobacter MLST was initially applied to distinguish between C. sakazakii and C. malonaticus because 16S rDNA sequencing is not always accurate enough, and biotyping is too subjective. [10] The Cronobacter MLST scheme uses 7 alleles; atpD , fusA , glnS , gltB , gyrB , infB and ppsA giving a concatenated sequence of 3036 bp for phylogenetic ...
An amplicon sequence template that has been prepared for amplification. The target sequence to be amplified is colored green. In molecular biology, an amplicon is a piece of DNA or RNA that is the source and/or product of amplification or replication events.