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Critical green inclusions, also known as green neutrophilic inclusions and informally, death crystals or crystals of death, [1] [2] are amorphous blue-green cytoplasmic inclusions found in neutrophils and occasionally in monocytes. They appear brightly coloured and refractile when stained with Wright-Giemsa stain.
The peripheral smear demonstrates mostly target cells, occasional hemoglobin C crystals, and only a few sickle cells. However, persons with hemoglobin SC disease (HbSC) have more significant retinopathy, ischemic necrosis of bone, and priapism than those with pure SS disease.
Blood film stained with Giemsa showing Plasmodium (center of image), the parasite that causes malaria infections.. In 1891 Romanowsky [8] [9] [10] developed a stain using a mixture of eosin (typically eosin Y) and aged solutions of methylene blue that formed hues unattributable to the staining components alone: distinctive shades of purple in the chromatin of the cell nucleus and within ...
It can be used for histopathological diagnosis of the Plasmodium species that cause malaria [2] and some other spirochete and protozoan blood parasites. It is also used to stain Wolbachia cells in host tissue. [3] Giemsa stain is a classic blood film stain for peripheral blood smears and bone marrow specimens.
Modern complete blood count analyzers can provide an automated white blood cell differential, but they have a limited ability to differentiate immature and abnormal cells, so manual examination of the blood smear is frequently indicated. [5] [6] Blood smear examination is the preferred diagnostic method for certain parasitic infections, such as ...
Wright's stain is a hematologic stain that facilitates the differentiation of blood cell types. It is classically a mixture of eosin (red) and methylene blue dyes. It is used primarily to stain peripheral blood smears, urine samples, and bone marrow aspirates, which are examined under a light microscope.
Heinz body stain of feline blood, showing three distinct Heinz bodies. Heinz bodies appear as small round inclusions within the red cell body, though they are not visible when stained with Romanowsky dyes. They are visualized more clearly with supravital staining [5] [6] (e.g., with new methylene blue, crystal violet or bromocresol green).
Plasmodium falciparum hemozoin crystals under polarised light. Plasmodium ovale microgametocyte in Giemsa-stained thin blood film, with annotated Schüffner's dots and hemozoin pigment. Haemozoin is a disposal product formed from the digestion of blood by some blood-feeding parasites.