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DESeq2 is a software package in the field of bioinformatics and computational biology for the statistical programming language R. It is primarily employed for the analysis of high-throughput RNA sequencing (RNA-seq) data to identify differentially expressed genes between different experimental conditions.
To quantile normalize two or more distributions to each other, without a reference distribution, sort as before, then set to the average (usually, arithmetic mean) of the distributions. So the highest value in all cases becomes the mean of the highest values, the second highest value becomes the mean of the second highest values, and so on.
The aim behind the choice of a variance-stabilizing transformation is to find a simple function ƒ to apply to values x in a data set to create new values y = ƒ(x) such that the variability of the values y is not related to their mean value.
fastqp Simple FASTQ quality assessment using Python. Kraken: [9] A set of tools for quality control and analysis of high-throughput sequence data. HTSeq [10] The Python script htseq-qa takes a file with sequencing reads (either raw or aligned reads) and produces a PDF file with useful plots to assess the technical quality of a run.
There are two main forms of normalization, namely data normalization and activation normalization. Data normalization (or feature scaling ) includes methods that rescale input data so that the features have the same range, mean, variance, or other statistical properties.
Feature scaling is a method used to normalize the range of independent variables or features of data. In data processing , it is also known as data normalization and is generally performed during the data preprocessing step.
Here's how to distinguish "sundowning"—agitation or confusion later in the day in dementia patients—from typical aging, from doctors who treat older adults.
Normalizing moments, using the standard deviation as a measure of scale. Coefficient of variation: Normalizing dispersion, using the mean as a measure of scale, particularly for positive distribution such as the exponential distribution and Poisson distribution.