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Supercritical fluid chromatography (SFC) [1] is a form of normal phase chromatography that uses a supercritical fluid such as carbon dioxide as the mobile phase. [2] [3] It is used for the analysis and purification of low to moderate molecular weight, thermally labile molecules and can also be used for the separation of chiral compounds.
Supercritical fluid chromatography (SFC) can be used on an analytical scale, where it combines many of the advantages of high performance liquid chromatography (HPLC) and gas chromatography (GC). It can be used with non-volatile and thermally labile analytes (unlike GC) and can be used with the universal flame ionization detector (unlike HPLC ...
Supercritical fluid chromatography is a separation technique in which the mobile phase is a fluid above and relatively close to its critical temperature and pressure. Specific techniques under this broad heading are listed below.
Gas chromatography (GC) Gas chromatography-mass spectrometry (GC-MS) ... Supercritical fluid chromatography (SFC) T. Transmission electron microscopy (TEM)
GC–MS (gas chromatography–mass spectrometry) LC–DAD (liquid chromatography–diode array detection) CE–MS (capillary electrophoresis–mass spectrometry) Chromatographic methods. HPLC (high performance liquid chromatography) GC (gas chromatography) UPLC (ultra performance liquid chromatography) Supercritical fluid chromatography ...
The supercritical solvent is passed into a vessel at lower pressure than the extraction vessel. The density, and hence dissolving power, of supercritical fluids varies sharply with pressure, and hence the solubility in the lower density CO 2 is much lower, and the material precipitates for collection. It is possible to fractionate the dissolved ...
Laboratory applications and examples [ edit ] Liquid-liquid extractions in the laboratory usually make use of a separatory funnel , where two immiscible phases are combined to separate a solute from one phase into the other, according to the relative solubility in each of the phases.
Supercritical fluid chromatography (SFC) Principle is very similar to that of HPLC. But SFC typically uses carbon dioxide as the mobile phase. Hence there is a need to pressurize the entire chromatographic flow path. SFC can differentiate enantiomers and enantiomer from the racemate; (+) from (-) and (±) Chiral capillary electrophoresis (CE) [45]
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