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  2. Bright-field microscopy - Wikipedia

    en.wikipedia.org/wiki/Bright-field_microscopy

    Bright-field microscopy ( BF) is the simplest of all the optical microscopy illumination techniques. Sample illumination is transmitted (i.e., illuminated from below and observed from above) white light, and contrast in the sample is caused by attenuation of the transmitted light in dense areas of the sample.

  3. Xylene - Wikipedia

    en.wikipedia.org/wiki/Xylene

    Xylene is used in the laboratory to make baths with dry ice to cool reaction vessels, [17] and as a solvent to remove synthetic immersion oil from the microscope objective in light microscopy. [18] In histology, xylene is the most widely used clearing agent. [19] Xylene is used to remove paraffin from dried microscope slides prior to staining.

  4. Condenser (optics) - Wikipedia

    en.wikipedia.org/wiki/Condenser_(optics)

    Condenser (optics) A condenser is an optical lens that renders a divergent light beam from a point light source into a parallel or converging beam to illuminate an object to be imaged. Condensers are an essential part of any imaging device, such as microscopes, enlargers, slide projectors, and telescopes. The concept is applicable to all kinds ...

  5. Oil immersion - Wikipedia

    en.wikipedia.org/wiki/Oil_immersion

    In light microscopy, oil immersion is a technique used to increase the resolving power of a microscope. This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index, thereby increasing the numerical aperture of the objective lens. Without oil, light waves reflect off the slide specimen ...

  6. Mirau interferometer - Wikipedia

    en.wikipedia.org/wiki/Mirau_interferometer

    A Mirau interferometer works on the same basic principle as a Michelson interferometer. The difference between the two is in the physical location of the reference arm. [1] The reference arm of a Mirau interferometer is located within a microscope objective assembly. It is named after André Henri Mirau, who filed a patent on the concept in 1949.

  7. Water immersion objective - Wikipedia

    en.wikipedia.org/wiki/Water_immersion_objective

    Water immersion objective. In light microscopy, a water immersion objective is a specially designed objective lens used to increase the resolution of the microscope. This is achieved by immersing both the lens and the specimen in water which has a higher refractive index than air, thereby increasing the numerical aperture of the objective lens.

  8. Optical microscope - Wikipedia

    en.wikipedia.org/wiki/Optical_microscope

    Optical microscope. The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century.

  9. Phase-contrast microscopy - Wikipedia

    en.wikipedia.org/wiki/Phase-contrast_microscopy

    Phase-contrast microscopy (PCM) is an optical microscopy technique that converts phase shifts in light passing through a transparent specimen to brightness changes in the image. Phase shifts themselves are invisible, but become visible when shown as brightness variations. When light waves travel through a medium other than a vacuum, interaction ...

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