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Köhler illumination is a method of specimen illumination used for transmitted and reflected light (trans- and epi-illuminated) optical microscopy.Köhler illumination acts to generate an even illumination of the sample and ensures that an image of the illumination source (for example a halogen lamp filament) is not visible in the resulting image.
Critical illumination generally has problems with evenness of illumination as an image of the illumination source (for example a halogen lamp filament) is visible in the resulting image. Köhler illumination has largely replaced critical illumination in modern scientific light microscopy although it requires additional optics which may not be ...
August Karl Johann Valentin Köhler (4 March 1866 – 12 March 1948) was a German professor and early staff member of Carl Zeiss AG in Jena, Germany.He is best known for his development of the microscopy technique of Köhler illumination, an important principle in optimizing microscopic resolution power by evenly illuminating the field of view.
The Arlow-Abbe condenser is a modified Abbe condenser that replaces the iris diaphragm, filter holder, lamp and lamp optics with a small OLED or LCD digital display unit. The display unit allows for digitally synthesised filters for dark-field, Rheinberg, oblique and dynamic (constantly changing) illumination under direct computer control.
A phase telescope or Bertrand lens is an optical device used in aligning the various optical components of a light microscope.In particular it allows observation of the back focal plane of the objective lens and its conjugated focal planes.
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Nearby, Kohler was in an airplane hangar, right in the center of Pearl Harbor, writing a letter to his mother on a typewriter when he heard an approaching aircraft continue to get closer and closer.
Bright-field microscopy (BF) is the simplest of all the optical microscopy illumination techniques. Sample illumination is transmitted (i.e., illuminated from below and observed from above) white light, and contrast in the sample is caused by attenuation of the transmitted light in dense areas of the sample. Bright-field microscopy is the ...