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[1] [2] Once stained as part of a sample, these organisms can resist the acid and/or ethanol-based decolorization procedures common in many staining protocols, hence the name acid-fast. [2] The mechanisms of acid-fastness vary by species although the most well-known example is in the genus Mycobacterium, which includes the species responsible ...
However, the mycolic acid in the cell wall of M. tuberculosis does not absorb the stain. Instead, acid-fast stains such as Ziehl–Neelsen stain, or fluorescent stains such as auramine are used. [4] Cells are curved rod-shaped and are often seen wrapped together, due to the presence of fatty acids in the cell wall that stick together. [11]
The two most common methods for visualizing these acid-fast bacilli as bright red against a blue background are the Ziehl-Neelsen stain and modified Kinyoun stain. Fite's stain is used to color M. leprae cells as pink against a blue background. Rapid Modified Auramine O Fluorescent staining has specific binding to slowly-growing mycobacteria ...
Mycobacterium leprae is an intracellular, pleomorphic, non-sporing, non-motile, acid-fast, pathogenic bacterium. [3] It is an aerobic bacillus (rod-shaped bacterium) with parallel sides and round ends, surrounded by the characteristic waxy coating of mycolic acid unique to mycobacteria .
NIOSH video on tuberculosis respiratory protection, 2002 During the HIV/AIDS epidemic in the US , up to 35% of those affected by TB were also infected by HIV. [ 125 ] Handling of TB-infected patients in US hospitals was known to create airborne TB that could infect others, especially in unventilated spaces.
The acid-fast staining method, in conjunction with auramine phenol staining, serves as the standard diagnostic tool and is widely accessible for rapidly diagnosing tuberculosis (caused by Mycobacterium tuberculosis) and other diseases caused by atypical mycobacteria, such as leprosy (caused by Mycobacterium leprae) and Mycobacterium avium ...
The Kinyoun method can be modified as a weak acid fast stain, which uses 0.5–1.0% sulfuric acid instead of hydrochloric acid.The weak acid fast stain, in addition to staining Mycobacteria, will also stain organisms that are not able to maintain the carbol fuchsin after decolorizing with HCl, such as Nocardia species and Cryptosporidium.
M. leprae is an aerobic, rod-shaped, acid-fast bacterium with a waxy cell envelope characteristic of the genus Mycobacterium. [34] M. leprae and M. lepromatosis are obligate intracellular pathogens and cannot grow or be cultured outside of host tissues. [5] [35] However, they can be grown using research animals such as mice and armadillos. [36 ...