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  2. Denaturation (biochemistry) - Wikipedia

    en.wikipedia.org/wiki/Denaturation_(biochemistry)

    In biochemistry, denaturation is a process in which proteins or nucleic acids lose folded structure present in their native state due to various factors, including application of some external stress or compound, such as a strong acid or base, a concentrated inorganic salt, an organic solvent (e.g., alcohol or chloroform), agitation and radiation, or heat. [3]

  3. Equilibrium unfolding - Wikipedia

    en.wikipedia.org/wiki/Equilibrium_unfolding

    In the less extensive technique of equilibrium unfolding, the fractions of folded and unfolded molecules (denoted as and , respectively) are measured as the solution conditions are gradually changed from those favoring the native state to those favoring the unfolded state, e.g., by adding a denaturant such as guanidinium hydrochloride or urea.

  4. Denaturing high performance liquid chromatography - Wikipedia

    en.wikipedia.org/wiki/Denaturing_High...

    This first step is followed by a step of denaturation–renaturation to create hetero- and homoduplexes from the two allele populations in the PCR. To find a homozygous polymorphism, proceed in the same way by premixing a DNA wild population to a population of polymorphic DNA to obtain heteroduplexes after the denaturation–renaturation step.

  5. Heat stabilization - Wikipedia

    en.wikipedia.org/wiki/Heat_stabilization

    Heat stabilization is an additive-free preservation technology for tissue samples which stops degradation and changes immediately and permanently. Heat stabilization uses rapid conductive heating, under controlled pressure, to generate a fast, homogeneous and irreversible thermal denaturation of proteins, resulting in a complete and permanent elimination of all enzymatic activity that would ...

  6. COLD-PCR - Wikipedia

    en.wikipedia.org/wiki/COLD-PCR

    Thus the denaturation can occur at the Tc, proceed to primer annealing, and then polymerase-mediated extension. Each round of amplification will include these three stages in that order. By utilizing the lower denaturation temperature, the reaction will discriminate toward the products with the lower Tm – i.e. the variant alleles.

  7. Head of US whistleblower office alleges Trump improperly ...

    www.aol.com/news/head-us-whistleblower-office...

    President Donald Trump fired the head of a U.S. watchdog agency focused on protecting government whistleblowers, the official said on Monday in a lawsuit alleging that his removal was unlawful.

  8. Trump administration ordered to restore removed CDC and FDA ...

    www.aol.com/trump-administration-ordered-restore...

    The temporary restraining order by U.S. District Judge John Bates in Washington, D.C. came in response to a lawsuit by the left-leaning medical advocacy group Doctors for America, which said the ...

  9. Serious Ozempic Side Effects, Confirmed: Here’s What to Know

    www.aol.com/serious-ozempic-side-effects...

    A study confirmed that side effects like pancreatitis and kidney damage are possible while taking GLP-1s like Ozempic. Here's what a doctor wants you to know.