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Micropropagation or tissue culture is the practice of rapidly multiplying plant stock material to produce many progeny plants, using modern plant tissue culture methods. [ 1 ] Micropropagation is used to multiply a wide variety of plants, such as those that have been genetically modified or bred through conventional plant breeding methods.
Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues, or organs under sterile conditions on a nutrient culture medium of known composition. It is widely used to produce clones of a plant in a method known as micropropagation .
Photoautotrophic tissue culture is defined as "micropropagation without sugar in the culture medium, in which the growth or accumulation of carbohydrates of cultures is dependent fully upon photosynthesis and inorganic nutrient uptake".
This technique is also called micropropagation. This is typically facilitated via use of a liquid, semi-solid, or solid growth medium, such as broth or agar. Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants.
Murashige and Skoog medium (or MSO or MS0 (MS-zero)) is the most popular plant growth medium used in the laboratories worldwide for cultivation of plant cell culture on agar. MS0 was invented by plant scientists Toshio Murashige and Folke K. Skoog in 1962 during Murashige's search for a new plant growth regulator. A number behind the letters MS ...
Plant propagation is the process by which new plants grow from various sources, including seeds, cuttings, and other plant parts. Plant propagation can refer to both man-made and natural processes. Plant propagation can refer to both man-made and natural processes.
The whole plants that are recovered can be used to experimentally determine gene function(s), or to enhance crop plant traits for modern agriculture. Callus is of particular use in micropropagation where it can be used to grow genetically identical copies of plants with desirable characteristics. To increase the yield, efficiency and explant ...
In plant tissue culture IBA and other auxins are used to initiate root formation in vitro in a procedure called micropropagation.Micropropagation of plants is the process of using small samples of plants called explants and causing them to undergo growth of differentiated or undifferentiated cells.