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Hematoxylin and eosin stain (or haematoxylin and eosin stain or hematoxylin-eosin stain; often abbreviated as H&E stain or HE stain) is one of the principal tissue stains used in histology. [ 1 ] [ 2 ] [ 3 ] It is the most widely used stain in medical diagnosis [ 1 ] and is often the gold standard . [ 4 ]
Hematoxylin staining shown as "basophilic" at top, seen with dual staining with hematoxylin and eosin (H&E). Haematoxylin stain is commonly followed (or counterstained) with another histologic stain, eosin. [10] [11] [1] When paired, this staining procedure is known as H&E staining, and is one of the most commonly used combinations in histology.
Immunohistochemistry stain versus hematoxylin counterstain. After immunohistochemical staining of the target antigen, another stain is often applied. The counterstain provide contrast that helps the primary stain stand out and makes it easier to examine the tissue morphology. It also helps with orientation and visualization of the tissue section.
The aim of staining is to reveal cellular components; counterstains are used to provide contrast. The most commonly used stain in histology is a combination of hematoxylin and eosin (often abbreviated H&E). Hematoxylin is used to stain nuclei blue, while eosin stains the cytoplasm and the extracellular connective tissue matrix of most cells ...
Phosphotungstic acid-haematoxylin staining demonstrating contraction band necrosis in an individual who had a myocardial infarction (heart attack). Phosphotungstic acid haematoxylin ( PTAH ) is a mix of haematoxylin with phosphotungstic acid , used in histology for staining .
It is similar to Masson's trichrome stain, but it uses Biebrich scarlet for the plasma stain. It was initially published by Ralph D. Lillie in 1940. [1] It is applied by submerging the fixated sample into the following three solutions: [2] Weigert's iron hematoxylin working solution, Biebrich scarlet solution, and Fast Green FCF solution.
A Ziehl–Neelsen stain is an acid-fast stain used to stain species of Mycobacterium tuberculosis that do not stain with the standard laboratory staining procedures such as Gram staining. This stain is performed through the use of both red coloured carbol fuchsin that stains the bacteria and a counter stain such as methylene blue .
Due to its short staining time, Diff-Quik stain is often used for initial screening of cytopathology specimens. This staining technique allows the cytotechnologist or pathologist to quickly assess the adequacy of the specimen, identify possible neoplastic or inflammatory changes, and decide whether or not additional staining is required. [4] [9 ...
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