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  2. Real-time polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Real-time_polymerase_chain...

    A real-time polymerase chain reaction (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time), not at its end, as in conventional PCR. Real-time PCR can be used ...

  3. Cepheid (company) - Wikipedia

    en.wikipedia.org/wiki/Cepheid_(company)

    It was the first amplified molecular diagnostic test using real-time PCR to receive this categorization. [14] This allowed the test to be performed by the over 27,000 institutions registered for CLIA Moderate Complexity in addition to the approximately 7,000 institutions registered for High Complexity tests. [citation needed]

  4. Relative fluorescence units - Wikipedia

    en.wikipedia.org/wiki/Relative_fluorescence_units

    The RFU measurements are used, for DNA profiling, in a real-time polymerase chain reaction (PCR). Two common methods for detection of products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labeled with a fluorescent reporter which permits detection only after ...

  5. Quantitative PCR instrument - Wikipedia

    en.wikipedia.org/wiki/Quantitative_PCR_instrument

    A quantitative PCR instrument [1] is a machine that amplifies and detects DNA. It combines the functions of a thermal cycler and a fluorimeter , enabling the process of quantitative PCR . The first quantitative PCR machine was described in 1993, [ 2 ] and two commercial models became available in 1996.

  6. Digital polymerase chain reaction - Wikipedia

    en.wikipedia.org/wiki/Digital_polymerase_chain...

    Chip-based Digital PCR (dPCR) is also a method of dPCR in which the reaction mix (also when used in qPCR) is divided into ~10,000 to ~45,000 partitions on a chip, then amplified using an endpoint PCR thermocycling machine, and is read using a high-powered camera reader with fluorescence filter (HEX, FAM, Cy5, Cy5.5 and Texas Red) for all ...

  7. Luminex Corporation - Wikipedia

    en.wikipedia.org/wiki/Luminex_Corporation

    The Luminex MultiCode technology is used for real-time polymerase chain reaction (PCR) and multiplexed PCR assays. [2] Luminex Corporation owns 315 issued patents worldwide, including over 124 issued patents in the United States based on its multiplexing xMAP platform.

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  9. Variants of PCR - Wikipedia

    en.wikipedia.org/wiki/Variants_of_PCR

    Quantitative Real-Time PCR (QRT-PCR), sometimes simply called Real-Time PCR (RT-PCR), refers to a collection of methods that use fluorescent dyes, such as Sybr Green, or fluorophore-containing DNA probes, such as TaqMan, to measure the amount of amplified product in real time as the amplification progresses.

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