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Primary cell culture is the ex vivo culture of cells freshly obtained from a multicellular organism, as opposed to the culture of immortalized cell lines.In general, primary cell cultures are considered more representative of in vivo tissues than cell lines, and this is recognized legally in some countries such as the UK (Human Tissue Act 2004). [1]
Critical green inclusions, also known as green neutrophilic inclusions and informally, death crystals or crystals of death, [1] [2] are amorphous blue-green cytoplasmic inclusions found in neutrophils and occasionally in monocytes. They appear brightly coloured and refractile when stained with Wright-Giemsa stain.
Cell culture is a fundamental component of tissue culture and tissue engineering, as it establishes the basics of growing and maintaining cells in vitro. The major application of human cell culture is in stem cell industry, where mesenchymal stem cells can be cultured and cryopreserved for future use. Tissue engineering potentially offers ...
If a cell line is thought to be contaminated, it is usually tested for authenticity. [b] The widespread contamination of HeLa cells was initially recognized by Walter Nelson-Rees using simple Giemsa stain karyotyping under a light microscope. This technique works well in recognizing HeLa because these cells have distinctive chromosome aberrations.
Necrosis (from Ancient Greek νέκρωσις (nékrōsis) 'death') is a form of cell injury which results in the premature death of cells in living tissue by autolysis. [1] The term "necrosis" came about in the mid-19th century and is commonly attributed to German pathologist Rudolf Virchow , who is often regarded as one of the founders of ...
Overview of signal transduction pathways involved in apoptosis. Cell death is the event of a biological cell ceasing to carry out its functions. This may be the result of the natural process of old cells dying and being replaced by new ones, as in programmed cell death, or may result from factors such as diseases, localized injury, or the death of the organism of which the cells are part.
Mycoplasma species are often found in research laboratories as contaminants in cell culture. Mycoplasmal cell culture contamination occurs due to contamination from individuals or contaminated cell culture medium ingredients. [34] Mycoplasma cells are physically small – less than 1 μm, so are difficult to detect with a conventional microscope.
A set of recommendations for describing the terminology of cell death was proposed by the Nomenclature Committee on Cell Death (NCCD) in 2009, because misusing words and concepts may slow down progress in the area of cell death research. [1] The classic definition of death defines it as a state characterized by the cessation of signs of life.