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Uranyl acetate staining is simple and quick to perform and one can examine the sample within a few minutes after staining. Some biological samples are not amenable to uranyl acetate staining and, in these cases, alternative staining techniques and or low-voltage electron microscopy technique may be more suitable.
Some suitable negative stains include ammonium molybdate, uranyl acetate, uranyl formate, phosphotungstic acid, osmium tetroxide, osmium ferricyanide [clarification needed] [2] and auroglucothionate. These have been chosen because they scatter electrons strongly and also adsorb to biological matter well.
Prostate cancer staging is the process by which physicians categorize the risk of cancer having spread beyond the prostate, or equivalently, the probability of being cured with local therapies such as surgery or radiation. Once patients are placed in prognostic categories, this information can contribute to the selection of an optimal approach ...
Prostate biopsy is a procedure in which small hollow needle-core samples are removed from a man's prostate gland to be examined for the presence of prostate cancer. It is typically performed when the result from a PSA blood test is high. [1] It may also be considered advisable after a digital rectal exam (DRE) finds possible abnormality.
Transrectal ultrasonography, or TRUS in short, is a method of creating an image of organs in the pelvis, most commonly used to perform an ultrasound-guided needle biopsy evaluation of the prostate gland in men with elevated prostate-specific antigen or prostatic nodules on digital rectal exam.
Negative staining is able to stain the background instead of the organisms because the cell wall of microorganisms typically has a negative charge which repels the negatively charged stain. The dyes used in negative staining are acidic. [1] Note: negative staining is a mild technique that may not destroy the microorganisms, and is therefore ...
There are several reasons why PIN is the most likely prostate cancer precursor. [3] PIN is more common in men with prostate cancer. High grade PIN can be found in 85 to 100% of radical prostatectomy specimens, [4] nearby or even in connection with prostate cancer. It tends to occur in the peripheral zone of the prostate.
Transrectal biopsy is a biopsy procedure in which a sample of tissue is removed from the prostate using a thin needle that is inserted through the rectum and into the prostate. [1] Transrectal ultrasound (TRUS) is usually used to guide the needle. [2] [3] The sample is examined under a microscope to see if it contains cancer.
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