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[4] Trypan Blue, a living-cell exclusion dye; Triphenyl tetrazolium chloride; Erythrosine, which is Red No. 3 in food coloring, can be used as an exclusion dye. 7-Aminoactinomycin D used e.g. in flowcytometric studies of hematopoietic stem cell viability. Other Vital Stains : Janus Green B is a basic dye and vital stain used in histology.
In vivo staining (also called vital staining or intravital staining) is the process of dyeing living tissues. By causing certain cells or structures to take on contrasting colours, their form or position within a cell or tissue can be readily seen and studied. The usual purpose is to reveal cytological details that might otherwise not be ...
Supravital stain of a smear of human blood from a patient with hemolytic anemia. The reticulocytes are the cells with the dark blue dots and curved linear structures (reticulum) in the cytoplasm. Supravital staining is a method of staining used in microscopy to examine living cells that
A "vital dye" or stain is a dye capable of penetrating living cells or tissues without causing immediate visible degenerative changes. [26] Such dyes are useful in medical and pathological fields in order to selectively color certain structures (such as cells) in order to distinguish them from surrounding tissue and thus make them more visible ...
Neutral red (toluylene red, Basic Red 5, or C.I. 50040) is a eurhodin dye used for staining in histology. It stains lysosomes red. [1] It is used as a general stain in histology, as a counterstain in combination with other dyes, and for many staining methods. Together with Janus Green B, it is used to stain embryonal tissues and supravital ...
Trypan blue is an azo dye.It is a direct dye for cotton textiles. [3] In biosciences, it is used as a vital stain to selectively colour dead tissues or cells blue.. Live cells or tissues with intact cell membranes are not coloured.
Janus Green B is a basic dye and vital stain used in histology. It is also used to stain mitochondria supravitally, as was introduced by Leonor Michaelis in 1900. [2] The indicator Janus Green B changes colour according to the amount of oxygen present. [3] When oxygen is present, the indicator oxidizes to a blue colour.
Light micrograph of a moss's leaf cells at 400X magnification. The following outline is provided as an overview of and topical guide to cell biology: . Cell biology – A branch of biology that includes study of cells regarding their physiological properties, structure, and function; the organelles they contain; interactions with their environment; and their life cycle, division, and death.