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Reference ranges for other molecules in CSF Substance Lower limit Upper limit Unit Corresponds to % of that in plasma Glucose: 50 [2] 80 [2] mg/dL ~60% [1] 2.2, [3] 2.8 [1] 3.9, [3] 4.4 [1] mmol/L Protein: 15 [1] [2] 40, [4] 45 [1] [2] mg/dL ~1% [1] Albumin: 7.8 [5] 40 [5] mg/dL: 0 [6] - 0.7% [6] - corresponding to an albumin (CSF/serum ...
Typically for an OCB analysis, the CSF is concentrated and the serum is diluted. After this dilution/concentration prealbumin appears as higher on CSF. Albumin is typically the dominant band on both fluids. Transferrin is another prominent protein on CSF column because its small molecular size easily increases its filtration in to CSF.
Serum protein electrophoresis (SPEP or SPE) is a laboratory test that examines specific proteins in the blood called globulins. [1] The most common indications for a serum protein electrophoresis test are to diagnose or monitor multiple myeloma , a monoclonal gammopathy of uncertain significance (MGUS), or further investigate a discrepancy ...
CSF albumin is a measurement used to determine the levels of albumin in cerebrospinal fluid. [1] A closely related test, CSF total protein is a measurement used to determine the levels of protein in cerebrospinal fluid. [2] [3] It combines the albumin, IgG, and other proteins. It can be useful in distinguishing among causes of Meningitis.
Cerebrospinal fluid (CSF) is a clear, colorless transcellular body fluid found within the meningeal tissue that surrounds the vertebrate brain and spinal cord, and in the ventricles of the brain. CSF is mostly produced by specialized ependymal cells in the choroid plexuses of the ventricles of the brain, and absorbed in the arachnoid granulations .
Proteins in the cerebrospinal fluid, normally albumin and globulin are present in the ratio of 8 to 1. Increases in protein levels are of diagnostic value in neurological diseases. The normal CSF is clear and transparent fluid. The Pandy's reaction makes it translucent or opaque.
14-3-3 proteins were initially found in brain tissue in 1967 and purified using chromatography and gel electrophoresis. In bovine brain samples, 14-3-3 proteins were located in the 14th fraction eluting from a DEAE-cellulose column and in position 3.3 on a starch electrophoresis gel. [6]
The high pH was chosen because antibodies are practically immobile at high pH. Electrophoresis equipment with a horizontal cooling plate was normally recommended for the electrophoresis. Immunoprecipitates are visible in the wet agarose gel, but are stained with protein stains like Coomassie brilliant blue in the dried gel.