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In molecular spectroscopy, a Jablonski diagram is a diagram that illustrates the electronic states and often the vibrational levels of a molecule, and also the transitions between them. The states are arranged vertically by energy and grouped horizontally by spin multiplicity . [ 1 ]
Jablonski diagram indicating intersystem crossing (left) and internal conversion (right). Internal conversion is a transition from a higher to a lower electronic state in a molecule or atom. [ 1 ] It is sometimes called "radiationless de-excitation", because no photons are emitted.
A Jablonski diagram describing the mechanism of triplet-triplet annihilation. The energy of the first triplet excited state (T 1) is transferred to a second triplet excited state (T 1), resulting in (1) the first T 1 returning to the singlet ground state S0 and (2) the second T 1 promoting to the singlet excited state (S 1).
English: Jablonski diagram of absorbance, non-radiative decay, and fluorescence. Electronic transitions are about 1 eV. Vibrational transitions are about 0.1 eV. Rotational transitions (not shown) are about 0.001 eV. Absorption is about 1 femtosecond, relaxation takes about 1 picosecond, fluorescence takes about 1 nanosecond.
Jablonski diagram of FRET with typical timescales indicated. The black dashed line indicates a virtual photon.. Förster resonance energy transfer (FRET), fluorescence resonance energy transfer, resonance energy transfer (RET) or electronic energy transfer (EET) is a mechanism describing energy transfer between two light-sensitive molecules (chromophores). [1]
Fluorescence is illustrated schematically with the classical Jablonski diagram, first proposed by Jabłoński in 1933 [2] to describe absorption and emission of light. In 1946, he settled in Toruń where he was appointed Head of the Faculty of Physics at the Nicolaus Copernicus University .
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Fluorescence microscopy relies upon fluorescent compounds, or fluorophores, in order to image biological systems.Since fluorescence and phosphorescence are competitive methods of relaxation, a fluorophore that undergoes intersystem crossing to the triplet excited state no longer fluoresces and instead remains in the triplet excited state, which has a relatively long lifetime, before ...