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Gram stain (Gram staining or Gram's method), is a method of staining used to classify bacterial species into two large groups: gram-positive bacteria and gram-negative bacteria. It may also be used to diagnose a fungal infection. [1] The name comes from the Danish bacteriologist Hans Christian Gram, who developed the technique in 1884. [2]
In particle physics, the electron mass (symbol: m e) is the mass of a stationary electron, also known as the invariant mass of the electron. It is one of the fundamental constants of physics . It has a value of about 9.109 × 10 −31 kilograms or about 5.486 × 10 −4 daltons , which has an energy-equivalent of about 8.187 × 10 −14 joules ...
In bacteriology, Gram-positive bacteria are bacteria that give a positive result in the Gram stain test, which is traditionally used to quickly classify bacteria into two broad categories according to their type of cell wall. The Gram stain is used by microbiologists to place bacteria into two main categories, Gram-positive (+) and Gram ...
The invariant mass of an electron is approximately 9.109 × 10 −31 kg, [80] or 5.489 × 10 −4 Da. Due to mass–energy equivalence, this corresponds to a rest energy of 0.511 MeV (8.19 × 10 −14 J). The ratio between the mass of a proton and that of an electron is about 1836.
A small intestine sample stained using the Grocott's methenamine silver stain demonstrating histoplasma (black round yeast with narrow budding) in a granuloma In pathology , the Grocott–Gömöri's methenamine silver stain , abbreviated GMS , is a popular staining method in histology .
The stain proved popular and in 1884 was used by Hans Christian Gram to stain bacteria. He credited Paul Ehrlich for the aniline-gentian violet mixture. [ 32 ] Grübler's gentian violet was probably very similar, if not identical, to Lauth's methyl violet, which had been used as a stain by Victor André Cornil in 1875.
Gram-positive cell walls are thick and the peptidoglycan (also known as murein) layer constitutes almost 95% of the cell wall in some Gram-positive bacteria and as little as 5-10% of the cell wall in Gram-negative bacteria. The peptidoglycan layer takes up the crystal violet dye and stains purple in the Gram stain.
They generally stain gram-positive, but as well as Bacillus, are often described as Gram-variable, because they show an increasing number of gram-negative cells as the culture ages. [11] The normal, reproducing cells of Clostridium, called the vegetative form, are rod-shaped, which gives them their name, from the Greek κλωστήρ or spindle.