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Hydrogen–deuterium exchange mass spectrometry (HX-MS or HDX-MS) can determine the overall deuterium content of molecules which have undergone H/D exchange. Because of the sample preparation required, it is typically considered to provide an accurate measurement of non-exchangeable hydrogen atoms only.
Hydrogen–deuterium exchange (HDX). ... The binding location of the antibody to the antigen can then be identified by mass spectrometry (MS). The cross-linked ...
Conventionally, mass spectrometry, such as gas chromatography-mass spectrometry and gas chromatography -time of flight, is a common technique for analyzing isotopically labeled molecules. [ 136 ] [ 137 ] This method involves ionizing and analyzing isotopologues of an intact organic molecule of interest rather than its products of pyrolysis or ...
HDX – Hydrogen/deuterium exchange; HCD – Higher-energy C-trap dissociation; I ... MS 2 – Mass spectrometry/mass spectrometry, i.e. tandem mass spectrometry;
It can be distinguished from normal hydrogen most easily by its mass, using mass spectrometry or infrared spectrometry. Deuterium can be detected by femtosecond infrared spectroscopy, since the mass difference drastically affects the frequency of molecular vibrations; 2 H–carbon bond vibrations are found in spectral regions free of other signals.
[1] [2] Mass spectra is a plot of relative abundance against mass-to-charge ratio. It is commonly used for the identification of organic compounds from electron ionization mass spectrometry. [3] [4] Organic chemists obtain mass spectra of chemical compounds as part of structure elucidation and the analysis is part of many organic chemistry ...
This latter method was adapted through the direct treatment of proteins and their complexes with hydroxyl radicals [4] [5] and can be generally denoted RP-MS (for Radical Probe - Mass Spectrometry) [6] akin to the designation used for Hydrogen-deuterium exchange Mass Spectrometry (denoted HD-MS or HX-MS).
Gross currently works on structural proteomics where he uses mass spectrometry in developing and implementing new approaches to protein footprinting, hydrogen/deuterium exchange, crosslinking and native MS.
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