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A set of standard 75 by 25 mm microscope slides. The white area can be written on to label the slide. A microscope slide (top) and a cover slip (bottom) A microscope slide is a thin flat piece of glass, typically 75 by 26 mm (3 by 1 inches) and about 1 mm thick, used to hold objects for examination under a microscope.
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The dimensions of the particles are usually measured from two-dimensional cross-sections or projections, as in a microscope field, but shape factors also apply to three-dimensional objects. The particles could be the grains in a metallurgical or ceramic microstructure , or the microorganisms in a culture , for example.
A microscopy slide scanner. Whole slide image quality comparison, with a slide scanned with a 20x objective and about 0.8 gigabytes (GB) in size to the left, and a 40x objective and approximately 1.2 GB in size to the right. Each image shows a red blood cell. Digital slides are created from glass slides using specialized scanning machines.
The shape and texture in each individual grain is made visible through the microscope. [ 7 ] As the microscopic scale covers any object that cannot be seen by the naked eye, yet is visible under a microscope, the range of objects that fall under this scale can be as small as an atom, visible underneath a transmission electron microscope . [ 8 ]
The microscope setup is based on an inverted microscope design. [ 2 ] [ 3 ] [ 4 ] An automated stage is used to record larger areas by mosaicing a series of single adjacent frames. The LED light is focused using a ball lens with a short focal length onto the sample surface in an oblique-angle cis-illumination scheme since standard microscopy ...
Moreover, live-cell imaging often employs special optical system and detector specifications. For example, ideally the microscopes used in live-cell imaging would have high signal-to-noise ratios, fast image acquisition rates to capture time-lapse video of extracellular events, and maintaining the long-term viability of the cells. [26]
One of the most important properties of microscope objectives is their magnification.The magnification typically ranges from 4× to 100×. It is combined with the magnification of the eyepiece to determine the overall magnification of the microscope; a 4× objective with a 10× eyepiece produces an image that is 40 times the size of the object.