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In this reaction an aromatic hydroxyl group is converted to the corresponding amine group. This is a reversible reaction. The first step in this reaction is an addition reaction of sodium bisulfite to an aromatic double bond. The Bucherer carbazole synthesis is a related organic reaction that uses sodium bisulfite as a reagent.
Figure 2: Outline of the chemical reaction that underlies the bisulfite-catalyzed conversion of cytosine to uracil. Bisulfite [1] sequencing (also known as bisulphite sequencing) is the use of bisulfite treatment of DNA before routine sequencing to determine the pattern of methylation.
Sodium bisulfite (or sodium bisulphite, sodium hydrogen sulfite) is a chemical mixture with the approximate chemical formula NaHSO 3. Sodium bisulfite is not a real compound, [ 2 ] but a mixture of salts that dissolve in water to give solutions composed of sodium and bisulfite ions.
The iodine clock reaction is a classical chemical clock demonstration experiment to display chemical kinetics in action; it was discovered by Hans Heinrich Landolt in 1886. [1] The iodine clock reaction exists in several variations, which each involve iodine species ( iodide ion, free iodine, or iodate ion) and redox reagents in the presence of ...
The Bucherer reaction in organic chemistry is the reversible conversion of a naphthol to a naphthylamine in the presence of ammonia and sodium bisulfite. [1] [2] [3 ...
The further reaction of the Schiff reagent with aldehydes is complex with several research groups reporting multiple reaction products with model compounds. In the currently accepted mechanism, the pararosaniline and bisulfite combine to yield the "decolorized" adduct with sulfonation at the central carbon as described and shown.
During amplification by polymerase chain reaction, uracil is converted to thymine. [1] Whole genome bisulfite sequencing is a next-generation sequencing technology used to determine the DNA methylation status of single cytosines by treating the DNA with sodium bisulfite before high-throughput DNA sequencing.
The technique is a variation of bisulfite sequencing, and combines bisulfite conversion based polymerase chain reaction with restriction digestion. Originally developed to reliably handle minute amounts of genomic DNA from microdissected paraffin -embedded tissue samples, the technique has since seen widespread usage in cancer research and ...