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Fluorescence lifetimes can be determined in the frequency domain by a phase-modulation method. The method uses a light source that is pulsed or modulated at high frequency (up to 500 MHz) such as an LED, diode laser or a continuous wave source combined with an electro-optic modulator or an acousto-optic modulator. The fluorescence is (a ...
A blood smear is made by placing a drop of blood on one end of a slide, and using a spreader slide to disperse the blood over the slide's length. The aim is to get a region, called a monolayer, where the cells are spaced far enough apart to be counted and differentiated.
In addition to increasing with average illumination intensity, the fusion frequency also increases with the extent of modulation (the maximal relative decrease in light intensity presented); for each frequency and average illumination, there is a characteristic modulation threshold, below which the flicker cannot be detected, and for each ...
This classification goes in the increasing order of wavelength, which is characteristic of the type of radiation. [1] There are no precisely defined boundaries between the bands of the electromagnetic spectrum; rather they fade into each other like the bands in a rainbow. Radiation of each frequency and wavelength (or in each band) has a mix of ...
Spectral sensitivity is the relative efficiency of detection, of light or other signal, as a function of the frequency or wavelength of the signal. In visual neuroscience, spectral sensitivity is used to describe the different characteristics of the photopigments in the rod cells and cone cells in the retina of the eye.
When the measurement involves two or more films in a stack of films, the theoretical expression for reflectance must be expanded to include the n(λ) and k(λ) spectra, plus thickness, t, of each film. However, the regression may not converge to unique values of the parameters, due to the non-linear nature of the expression for reflectance.
blood film in a patient with hereditary elliptocytosis: approximately 60% to 70% of the RBCs are elliptocytes. Elliptocyte compared to other forms of poikilocytosis . Elliptocytes , also known as ovalocytes or cigar cells , are abnormally shaped red blood cells that appear oval or elongated, from slightly egg-shaped to rod or pencil forms.
Most red blood cells are released into the blood as reticulocytes. Polychromasia occurs when the immature reticulocytes of the bone marrow are released, resulting in a grayish blue color of the cells. This color is seen because of the ribosomes still left on the immature blood cells, which are not found on mature red blood cells.