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An assay (analysis) is never an isolated process, as it must be accompanied with pre- and post-analytic procedures. Both the communication order (the request to perform an assay plus related information) and the handling of the specimen itself (the collecting, documenting, transporting, and processing done before beginning the assay) are pre-analytic steps.
On the wet basis the value is the ratio of the weight of water to the total weight of the solution (1 / 5 = 20% in the example), so the moisture content is always below 100% (in the previous examples the moisture content was specified on this "moisture content wet basis"). For the moisture content dry basis the ratio of the weight of the water ...
The Bradford protein assay (also known as the Coomassie protein assay) was developed by Marion M. Bradford in 1976. [1] It is a quick and accurate [2] spectroscopic analytical procedure used to measure the concentration of protein in a solution. The reaction is dependent on the amino acid composition of the measured proteins.
If the measured response is binary, the assay is quantal; if not, it is quantitative. [3] A bioassay may be used to detect biological hazards or to give an assessment of the quality of a mixture. [4] A bioassay is often used to monitor water quality as well as wastewater discharges and its impact on the surroundings. [5]
It is less perturbative because the sample is not dried onto a surface, this drying process is often done in negative-stain TEM, and because Cryo-EM does not require contrast agent like heavy metal salts (e.g. uranyl acetate or phoshotungstic acid) which also may affect the structure of the biomolecule. Transmission electron microscopy, as a ...
Gasoline, Bunsen burners, and crucibles may also be used to evaporate and isolate substances in their dry forms. [4] [5] Wet chemistry is not performed with any advanced instruments since most automatically scan substances. [6] Although, simple instruments such as scales are used to measure the weight of a substance before and after a change ...
The disk diffusion test (also known as the agar diffusion test, Kirby–Bauer test, disc-diffusion antibiotic susceptibility test, disc-diffusion antibiotic sensitivity test and KB test) is a culture-based microbiology assay used in diagnostic and drug discovery laboratories. In diagnostic labs, the assay is used to determine the susceptibility ...
The purpose of this technique is to analyze the activity of a gene transcription promoter (in terms of expression of a so-called reporter gene under the regulatory control of that promoter) either in a quantitative manner, involving some measure of activity, or qualitatively (on versus off) through visualization of its activity in different cells, tissues, or organs.