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HN1 (bis(2-chloroethyl)ethylamine), a DNA crosslinker. Like most crosslinkers, this molecule has two reactive groups. Intrastrand DNA crosslinks have strong effects on organisms because these lesions interfere with transcription and replication. These effects can be put to good use (addressing cancer) or they can be lethal to the host organism.
Mitomycin C can only act as a crosslinker when a DNA nucleotide has had a reduction to its quinone ring. When two dG's have been rearranged and methylated in this manner, a 5'-GC interstrand crosslink can be formed with the exo amines of each nucleobase.
A spacer arm, generally consisting of several atoms, separates the two molecules, and the nature and length of this spacer is important to consider when designing an assay involving the selected crosslinker. Bissulfosuccinimidyl suberate is an example of a homobifunctional crosslinker. [3]
Disuccinimidyl suberate (DSS) is a six-carbon lysine-reactive non-cleavable cross-linking agent.. It consists of functional groups It is a homobifunctional N-hydroxysuccinimide (NHS) ester formed by carbodiimide-activation of carboxylate molecules, with identical reactive groups at either end. [2]
Glutaraldehyde is used in biochemistry applications as an amine-reactive homobifunctional crosslinker and fixative. [7] [8] It kills cells quickly by crosslinking their proteins. It is usually employed alone or mixed with formaldehyde [9] as the first of two fixative processes to stabilize specimens such as bacteria, plant material, and human ...
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EDC is a water-soluble carbodiimide reagent used for a wide range of purposes. Apart from uses similar to those of DCC and DIC, it is also used for various biochemical purposes as a crosslinker or chemical probe.
Kamala Harris' Bungled Answer On ‘The View’ About Biden Seen As Turning Point For Campaign. During a live interview on the show in October, co-host Sunny Hostin asked the vice president if ...