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Bromothymol blue (also known as bromothymol sulfone phthalein and BTB) is a pH indicator. It is mostly used in applications that require measuring substances that would have a relatively neutral pH (near 7). A common use is for measuring the presence of carbonic acid in a liquid.
Bromothymol blue was added in order to reduce false positives. The citrate agar is green before inoculation, and turns blue, because of BTB as a positive test indicator, meaning citrate is utilized. The test is also prepared on a slant to maximize bacterial growth for an even better indication of the use of citrate.
Bacteria are inoculated on a medium containing sodium citrate and a pH indicator such as bromothymol blue. The medium also contains inorganic ammonium salts, which are utilized as sole source of nitrogen. Use of citrate involves the enzyme citrate lyase, which breaks down citrate to oxaloacetate and acetate.
If the tube with oil doesn't turn yellow, but the open tube does turn yellow, then the bacterium is oxidizing glucose. If the tube with mineral oil doesn't change, and the open tube turns blue, then the organism neither ferments, nor oxidizes glucose. Instead it oxidizes peptones, which liberates ammonia, turning the indicator blue.
Critics have long argued that while studying the effects of Red Dye No. 3 in humans poses ethical and scientific challenges, its ban in cosmetics should have logically extended to the food supply.
Use of citrate results in the creation of carbonates and bicarbonates as byproducts. Organisms degrading citrate must also use the ammonium salts, producing ammonia, [10] thus increasing the pH of the medium. [11] The increase in pH then causes color change in the bromothymol blue indicator, turning it blue.
The Mayo Clinic diet, a program that adheres to this notion, was developed by medical professionals based on scientific research, so you can trust that this program is based on science, and not ...
Supplement Side Effects Earlier this year, social media was flooded with creators trying the sleepy girl mocktail , a concoction consisting of tart cherry juice and magnesium powder, both of which ...
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