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Resolution in the context of structural biology is the ability to distinguish the presence or absence of atoms or groups of atoms in a biomolecular structure. Usually, the structure originates from methods such as X-ray crystallography, electron crystallography, or cryo-electron microscopy. The resolution is measured of the "map" of the ...
Two-photon excitation microscopy of mouse intestine.Red: actin.Green: cell nuclei.Blue: mucus of goblet cells.Obtained at 780 nm using a Ti-sapphire laser.. Two-photon excitation microscopy (TPEF or 2PEF) is a fluorescence imaging technique that is particularly well-suited to image scattering living tissue of up to about one millimeter in thickness.
The aspect ratio of a geometric shape is the ratio of its sizes in different dimensions. For example, the aspect ratio of a rectangle is the ratio of its longer side to its shorter side—the ratio of width to height, [1][2] when the rectangle is oriented as a "landscape". The aspect ratio is most often expressed as two integer numbers ...
In 1964, Adam S. G. Curtis coined the term Interference Reflection Microscopy (IRM), using it in the field of cell biology to study embryonic chick heart fibroblasts. [1][2] He used IRM to look at adhesion sites and distances of fibroblasts, noting that contact with the glass was mostly limited to the cell periphery and the pseudopodia. [1]
The ratio of the focal length of the objective and the eyepiece, when mounted in a standard tube length, gives an approximate magnification of the system. Due to their design, compound microscopes have improved resolving power and contrast in comparison to simple microscopes, [ 11 ] and can be used to view the structure, shape and motility of a ...
Super-resolution microscopy. Super-resolution microscopy is a series of techniques in optical microscopy that allow such images to have resolutions higher than those imposed by the diffraction limit, [1][2] which is due to the diffraction of light. [3] Super-resolution imaging techniques rely on the near-field (photon-tunneling microscopy [4 ...
Macro photography. Macro photograph of a common yellow dung fly (Scathophaga stercoraria) made using a lens at its maximum 1:1 reproduction ratio, and an 18×24mm image sensor, the on-screen display of the photograph results in a greater than life-size image. Headshot of a dragonfly taken with a 100 mm macro lens coupled with a 50 mm lens in ...
Cryogenic electron tomography (cryoET) is an imaging technique used to reconstruct high-resolution (~1–4 nm) three-dimensional volumes of samples, often (but not limited to) biological macromolecules and cells. [1][2] cryoET is a specialized application of transmission electron cryomicroscopy (CryoTEM) in which samples are imaged as they are ...