enow.com Web Search

Search results

  1. Results from the WOW.Com Content Network
  2. Denaturation (biochemistry) - Wikipedia

    en.wikipedia.org/wiki/Denaturation_(biochemistry)

    In biochemistry, denaturation is a process in which proteins or nucleic acids lose folded structure present in their native state due to various factors, including application of some external stress or compound, such as a strong acid or base, a concentrated inorganic salt, an organic solvent (e.g., alcohol or chloroform), agitation and radiation, or heat. [3]

  3. Hyperchromicity - Wikipedia

    en.wikipedia.org/wiki/Hyperchromicity

    The most famous example is the hyperchromicity of DNA that occurs when the DNA duplex is denatured. [1] The UV absorption is increased when the two single DNA strands are being separated, either by heat or by addition of denaturant or by increasing the pH level. The opposite, a decrease of absorbance is called hypochromicity.

  4. Nucleic acid thermodynamics - Wikipedia

    en.wikipedia.org/wiki/Nucleic_acid_thermodynamics

    Nucleic acid thermodynamics is the study of how temperature affects the nucleic acid structure of double-stranded DNA (dsDNA). The melting temperature (T m) is defined as the temperature at which half of the DNA strands are in the random coil or single-stranded (ssDNA) state. T m depends on the length of the DNA molecule and its specific ...

  5. DNA - Wikipedia

    en.wikipedia.org/wiki/DNA

    The A form occurs under non-physiological conditions in partly dehydrated samples of DNA, while in the cell it may be produced in hybrid pairings of DNA and RNA strands, and in enzyme-DNA complexes. [ 54 ] [ 55 ] Segments of DNA where the bases have been chemically modified by methylation may undergo a larger change in conformation and adopt ...

  6. Southern blot - Wikipedia

    en.wikipedia.org/wiki/Southern_blot

    Denaturation: If alkaline transfer methods are used, the DNA gel is placed into an alkaline solution (typically containing sodium hydroxide) to denature the double-stranded DNA. The denaturation in an alkaline environment may improve binding of the negatively charged thymine residues of DNA to a positively charged amino groups of membrane ...

  7. Nucleic acid structure - Wikipedia

    en.wikipedia.org/wiki/Nucleic_acid_structure

    A-DNA, is a form of the DNA duplex observed under dehydrating conditions. It is shorter and wider than B-DNA. RNA adopts this double helical form, and RNA-DNA duplexes are mostly A-form, but B-form RNA-DNA duplexes have been observed. [14] In localized single strand dinucleotide contexts, RNA can also adopt the B-form without pairing to DNA. [15]

  8. Nucleic acid metabolism - Wikipedia

    en.wikipedia.org/wiki/Nucleic_acid_metabolism

    The other purine nucleoside, guanosine, is cleaved to form guanine. Guanine is then deaminated via guanine deaminase to form xanthine which is then converted to uric acid. Oxygen is the final electron acceptor in the degradation of both purines. Uric acid is then excreted from the body in different forms depending on the animal. [5]

  9. Sodium hydroxide - Wikipedia

    en.wikipedia.org/wiki/Sodium_hydroxide

    NaOH and its monohydrate form orthorhombic crystals with the space groups Cmcm and Pbca (oP24), respectively. The monohydrate cell dimensions are a = 1.1825, b = 0.6213, c = 0.6069 nm . The atoms are arranged in a hydrargillite -like layer structure, with each sodium atom surrounded by six oxygen atoms, three each from hydroxide ions and three ...