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The Meselson-Stahl experiment was a landmark experiment in molecular biology that provided evidence for the semiconservative replication of DNA. Conducted in 1958 by Matthew Meselson and Franklin Stahl , the experiment involved growing E. coli bacteria in a medium containing heavy isotope of nitrogen ( 15 N) for several generations.
Scheme of the principle of the FISH Experiment to localize a gene in the nucleus. First, a probe is constructed. The probe must be large enough to hybridize specifically with its target but not so large as to impede the hybridization process. The probe is tagged directly with fluorophores, with targets for antibodies or with biotin.
Molecular biology: DNA sequencer: Used to automate the DNA sequencing process: Genetics, Molecular biology: Enzyme-linked immunosorbent assay (ELISA) Used to detect the presence of a ligand (commonly a protein) in a liquid sample using antibodies directed against the protein to be measure: Biochemistry, Molecular biology: Gene knockout
Stentor coeruleus, used in molecular biology (its genome has been sequenced), [5] and is studied as a model of single-cell regeneration.; Dictyostelium discoideum, used in molecular biology and genetics (its genome has been sequenced), and is studied as an example of cell communication, differentiation, and programmed cell death.
In molecular biology, mutagenesis is an important laboratory technique whereby DNA mutations are deliberately engineered to produce libraries of mutant genes, proteins, strains of bacteria, or other genetically modified organisms. The various constituents of a gene, as well as its regulatory elements and its gene products, may be mutated so ...
Molecular biology techniques are common methods used in molecular biology, biochemistry, genetics and biophysics which generally involve manipulation and analysis of DNA, RNA, protein, and lipid Wikimedia Commons has media related to Molecular biology techniques .
Methods in Molecular Biology is a book series published by Humana Press (an imprint of Springer Science+Business Media) that covers molecular biology research methods and protocols. The book series was introduced by series editor John M. Walker in 1983 and provides step-by-step instructions for carrying out experiments in a research lab. [1]
The methods in this section are primarily computational although they typically require data generated by wet lab experiments. Protein–protein docking , the prediction of protein–protein interactions based only on the three-dimensional protein structures from X-ray diffraction of protein crystals might not be satisfactory.