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DMEM (Dulbecco's modified Eagle's medium) was originally suggested as Eagle's medium with a 'fourfold concentration of amino acids and vitamins' by Renato Dulbecco and G. Freeman published in 1959. [5] The commercial versions of this medium have additional modifications, see an example in the table below. [6]
The constituents of a chemically defined media include: a basal media (such as DMEM, F12, or RPMI 1640, containing amino acids, vitamins, inorganic salts, buffers, antioxidants and energy sources), which is supplemented with recombinant albumin, chemically defined lipids, recombinant insulin and/or zinc, recombinant transferrin or iron ...
This page was last edited on 31 January 2009, at 15:27 (UTC).; Text is available under the Creative Commons Attribution-ShareAlike 4.0 License; additional terms may apply.
Tissue culture flasks. RPMI 1640, simply known as RPMI medium, is a cell culture medium commonly used to culture mammalian cells. [1] RPMI 1640 was developed by George E. Moore, Robert E. Gerner, and H. Addison Franklin in 1966 at Roswell Park Comprehensive Cancer Center (formerly known as Roswell Park Memorial Institute), from where it derives its name. [2]
A bottle of DMEM cell culture medium. Aside from temperature and gas mixture, the most commonly varied factor in culture systems is the cell growth medium. Recipes for growth media can vary in pH, glucose concentration, growth factors, and the presence of other nutrients.
DMEM Dulbecco's Modified Eagle's Medium; Eagle's minimal essential medium (a minimum ingredient cell culture medium) Fermentation (food) GMEM (Glasgow's Minimal Essential Medium) Murashige and Skoog medium (Plant cell culture medium) RPMI (Roswell Park Memorial Institute medium), for lymph cells
MEF cells can be cultured in vitro in DMEM medium with 10% FBS. To transmit MEFs, researches should use trypsin to digest the cells (making them detach from the surface) and transmit 1/5 cells digested into a new dish.
4T1 cell culture - 50% confluence. 4T1 is a breast cancer cell line derived from the mammary gland tissue of a mouse BALB/c strain. [1] 4T1 cells are epithelial and are resistant to 6-thioguanine. [1]