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Bial's reagent consists of 0.4 g orcinol, 200 ml of concentrated hydrochloric acid and 0.5 ml of a 10% solution of ferric chloride. [2] Bial's test is used to distinguish pentoses from hexoses; this distinction is based on the color that develops in the presence of orcinol and iron (III) chloride. Furfural from pentoses gives a blue or green color.
Manfred Bial (10 December 1869 – 26 May 1908) was a German physician who invented a test for pentoses using orcinol, now known as Bial's test. [1] Bial was born on 10 December 1869 in Breslau, the son of Max Bial. He was an assistant at the Kaiserin-Augusta-Hospital in Berlin. Bial died on 26 May 1908 in Monaco. [citation needed]
Bial's test; References This page was last edited on 17 July 2024, at 09:05 (UTC). Text is available under the Creative Commons Attribution-ShareAlike 4.0 License ...
It is used in the production of the dye orcein and as a reagent in some chemical tests for pentoses, such as Bial's Test. It may be synthesized from toluene; more interesting is its production when acetone dicarboxylic ester is condensed with the aid of sodium. It crystallizes in colorless prisms with one molecule of water, which redden on ...
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Enterococcus colonies (black) growing on BEA. Bile salts are the selective ingredient, while esculin is the differential component. Enterococcus hydrolyze esculin to products that react with ferric citrate in the medium to produce insoluble iron salts, resulting in the blackening of the medium.
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