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Sorbitol-MacConkey agar. Sorbitol-MacConkey agar is a variant of traditional MacConkey agar used in the detection of E. coli O157:H7. [1] Traditionally, MacConkey agar has been used to distinguish those bacteria that ferment lactose from those that do not. This is important because gut bacteria, such as Escherichia coli, can typically ferment ...
MacConkey agar is a selective and differential culture medium for bacteria. It is designed to selectively isolate gram-negative and enteric (normally found in the intestinal tract) bacteria and differentiate them based on lactose fermentation. [1] Lactose fermenters turn red or pink on MacConkey agar, and nonfermenters do not change color.
Sorbitol (/ ˈsɔː (r) bɪtɒl /), less commonly known as glucitol (/ ˈɡluːsɪtɒl /), is a sugar alcohol with a sweet taste which the human body metabolizes slowly. It can be obtained by reduction of glucose, which changes the converted aldehyde group (−CHO) to a primary alcohol group (−CH 2 OH). Most sorbitol is made from potato ...
The reaction steps are: hydrogenation of D - glucose to D - sorbitol, an organic reaction with nickel as a catalyst under high temperature and high pressure. Microbial oxidation or fermentation of sorbitol to L - sorbose with acetobacter [2] at pH 4-6 and 30 °C. The final step is a ring-closing step or gamma lactonization with removal of water ...
Cystine tryptic agar (CTA), also known as cystine trypticase agar, [1][2] is a growth medium used for the identification of microorganisms. [3] It can be used to determine if organisms can ferment various carbohydrates, including maltose, lactose, and sucrose. This approach can be used to type organisms because although strains quickly gain ...
Composition. The TSI slant is a test tube that contains agar, a pH-sensitive dye (phenol red), 1% lactose, 1% sucrose, 0.1% glucose, [2] and sodium thiosulfate and ferrous sulfate or ferrous ammonium sulfate. All of these ingredients are mixed together, heated to sterility, and allowed to solidify in the test tube at a slanted angle.
A stool culture can detect the bacterium. The sample is cultured on sorbitol-MacConkey (SMAC) agar, or the variant cefixime potassium tellurite sorbitol-MacConkey agar (CT-SMAC [15]). On SMAC agar, O157:H7 colonies appear clear due to their inability to ferment sorbitol, while the colonies of the usual sorbitol-fermenting serotypes of E. coli ...
Solid state fermentation (SSF) is a biomolecule manufacturing process used in the food, pharmaceutical, cosmetic, fuel and textile industries. These biomolecules are mostly metabolites generated by microorganisms grown on a solid support selected for this purpose. This technology for the culture of microorganisms is an alternative to liquid or ...