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β-Galactosidase (EC 3.2.1.23, beta-gal or β-gal; ... It is commonly used in molecular biology as a reporter marker to monitor gene expression.
Colonies that produce β-galactosidase are turned blue by X-gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside) which is an artificial substrate for B-galactosidase whose cleavage results in galactose and 4-Cl,3-Br indigo thus producing a deep blue color. [16] Allolactose is an isomer of lactose and is the inducer of the lac operon. Lactose is ...
Lack of alpha-galactosidase activity in leukocytes has been linked to Fabry Disease. [2] Galactosidases have a variety of uses, including the production of prebiotics, the biosynthesis of transgalactosylated products, and the removal of lactose. B-galactosidase forms the basis of lac z operon in bacteria which can be used to control gene ...
Senescence-associated beta-galactosidase (SA-β-gal or SABG) is a hypothetical hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides. Senescence-associated beta-galactosidase, along with p16 Ink4A , is regarded to be a biomarker of cellular senescence .
Galactosidase, beta 1, also known as GLB1, is a protein which in humans is encoded by the GLB1 gene. [ 5 ] [ 6 ] The GLB1 protein is a beta-galactosidase that cleaves the terminal beta- galactose from ganglioside substrates and other glycoconjugates. [ 7 ]
A schematic representation of the molecular mechanism involved for screening recombinant cells. The lacZ fragment, whose synthesis can be induced by IPTG, is capable of intra-allelic complementation with a defective form of β-galactosidase enzyme encoded by host chromosome (mutation lacZDM15 in E. coli JM109, DH5α and XL1-Blue strains). [4]
A common reporter in bacteria is the E. coli lacZ gene, which encodes the protein beta-galactosidase. [10] This enzyme causes bacteria expressing the gene to appear blue when grown on a medium that contains the substrate analog X-gal .
2717 11605 Ensembl ENSG00000102393 ENSMUSG00000031266 UniProt P06280 P51569 RefSeq (mRNA) NM_000169 NM_013463 RefSeq (protein) NP_000160 NP_038491 Location (UCSC) Chr X: 101.39 – 101.41 Mb Chr X: 133.49 – 133.5 Mb PubMed search Wikidata View/Edit Human View/Edit Mouse Galactosidase alpha is an enzyme that in humans is encoded by the GLA gene. Two recombinant forms of human α-galactosidase ...