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An electropherogram (also called electrophoretogram, sequencing chromatogram, EPG, and e-gram) is a record or chart produced when electrophoresis is used in an analytical technique, primarily in the fields of forensic biology, molecular biology, and biochemistry. [1]
Mass spectrometry is a scientific technique for measuring the mass-to-charge ratio of ions. It is often coupled to chromatographic techniques such as gas-or liquid chromatography and has found widespread adoption in the fields of analytical chemistry and biochemistry where it can be used to identify and characterize small molecules and proteins ().
A mass chromatogram is a representation of mass spectrometry data as a chromatogram, where the x-axis represents time and the y-axis represents signal intensity. [1] The source data contains mass information; however, it is not graphically represented in a mass chromatogram in favor of visualizing signal intensity versus time.
Two resolved peaks in a chromatogram. The theoretical plate height is given by = where L is the column length and N the number of theoretical plates. [5] The relation between plate number and peak width at the base is given by = ().
Tableau Software, LLC is an American interactive data visualization software company focused on business intelligence. [ 2 ] [ 3 ] It was founded in 2003 in Mountain View, California , and is currently headquartered in Seattle, Washington . [ 4 ]
It means that to increase resolution of two peaks on a chromatogram, one of the three terms of the equation need to be modified. 1) N can be increased by lengthening the column (least effective, as doubling the column will get a 2 1/2 or 1.44x increase in resolution).
In the eyes of Nate Rosen, a consumer packaged goods expert, the variety of coffee creamers has “gone off the rails in the best way possible.”
SRM can be used for targeted quantitative proteomics by mass spectrometry. [6] Following ionization in, for example, an electrospray source, a peptide precursor is first isolated to obtain a substantial ion population of mostly the intended species.