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Escherichia coli BL21(DE3) is a commonly used protein production strain of the E. coli bacterium. This strain combines several features that allow for excessive expression of heterologous proteins . It is derived from the B lineage of E. coli .
DH5-Alpha Cells are E. coli cells engineered by American biologist Douglas Hanahan to maximize transformation efficiency. They are defined by three [ 1 ] mutations: recA1, endA1 which help plasmid insertion and lacZΔM15 which enables blue white screening .
Enterohemorrhagic E. coli (EHEC) Enteroaggregative E. coli (EAEC) Uropathogenic E. coli (UPEC) Verotoxin-producing E. coli; E. coli O157:H7 is an enterohemorrhagic strain also 2006 North American E. coli outbreak; E. coli O104:H4, also 2011 E. coli O104:H4 outbreak; Escherichia coli O121; Escherichia coli O104:H21; Escherichia coli K1, meningitis
Escherichia coli bacterium, 2021, Illustration by David S. Goodsell, RCSB Protein Data Bank This painting shows a cross-section through an Escherichia coli cell. The characteristic two-membrane cell wall of gram-negative bacteria is shown in green, with many lipopolysaccharide chains extending from the surface and a network of cross-linked ...
E. coli colonies containing the fluorescent pGLO plasmid. Escherichia coli (/ ˌ ɛ ʃ ɪ ˈ r ɪ k i ə ˈ k oʊ l aɪ /; commonly abbreviated E. coli) is a Gram-negative gammaproteobacterium commonly found in the lower intestine of warm-blooded organisms (endotherms). The descendants of two isolates, K-12 and B strain, are used routinely in ...
Overview of the use of the SOS response for genotoxicity testing. The SOS chromotest is a biological assay to assess the genotoxic potential of chemical compounds. The test is a colorimetric assay which measures the expression of genes induced by genotoxic agents in Escherichia coli, by means of a fusion with the structural gene for β-galactosidase.
Optimized strains of E. coli are typically used for this application. E. coli are also often used as a chassis for the expression of simple proteins. These strains, such as BL21, are genetically modified to minimize protease activity, hence enabling potential for high efficiency industrial scale protein production. [10]
The vector is then inserted into a competent host cell viable for transformation, which are then grown in the presence of X-gal. Cells transformed with vectors containing recombinant DNA will produce white colonies; cells transformed with non-recombinant plasmids (i.e. only the vector) grow into blue colonies.