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Thick elastic fibers from the visceral pleura (outer lining) of the human lung. Elastic fibers are found in the skin, lungs, arteries, veins, connective tissue proper, elastic cartilage, periodontal ligament, fetal tissue and other tissues which must undergo mechanical stretching. [1] In the lung there are thick and thin elastic fibers. [3]
Connective tissue is found in between other tissues everywhere in the body, including the nervous system. The three meninges, membranes that envelop the brain and spinal cord, are composed of connective tissue. Most types of connective tissue consists of three main components: elastic and collagen fibers, ground substance, and cells. [2]
Elastic fiber in the body is a mixture of amorphous elastin and fibrous fibrillin. Both components are primarily made of smaller amino acids such as glycine , valine , alanine , and proline . [ 11 ] [ 14 ] The total elastin ranges from 58 to 75% of the weight of the dry defatted artery in normal canine arteries. [ 15 ]
The collagen fibers are approximately 1-2 μm thick. Thus, the resolution of the imaging technique needs to be approximately 0.5 μm. Some techniques allow the direct acquisition of volume data while other need the slicing of the specimen. In both cases, the volume that is extracted must be able to follow the fiber bundles across the volume.
The dermis is composed of three major types of cells: [3] fibroblasts, macrophages, and mast cells.. Apart from these cells, the dermis is also composed of matrix components such as collagen (which provides strength), elastin (which provides elasticity), and extrafibrillar matrix, an extracellular gel-like substance primarily composed of glycosaminoglycans (most notably hyaluronan ...
Link proteins such as vinculin, spectrin and actomyosin stabilize the proteoglycans and organize elastic fibers in the ECM. [2] Changes in the density of ground substance can allow collagen fibers to form aberrant cross-links. [2] Loose connective tissue is characterized by few fibers and cells, and a relatively large amount of ground substance.
Its fibers run in random directions and are mostly collagenous, but elastic and reticular fibers are also present. Areolar tissue is highly variable in appearance. In many serous membranes , it appears as a loose arrangement of collagenous and elastic fibers, scattered cells of various types; abundant ground substance ; numerous blood vessels.
Weigert's elastic stain is a combination of stains used in histology which is useful in identifying elastic fibers. Often orcein or a combination of resorcinol and fuchsine are used for staining. For counterstaining cell nuclei nuclear fast red or hematoxylin is also used. After applying, elastic fibers show up blue colored while cell nuclei ...