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The oxidase test is used to determine whether an organism possesses the cytochrome c oxidase enzyme. The test is used as an aid for the differentiation of Neisseria , Moraxella , Campylobacter and Pasteurella species (oxidase positive).
In biochemistry, an oxidase is an oxidoreductase (any enzyme that catalyzes a redox reaction) that uses dioxygen (O 2) as the electron acceptor. In reactions involving donation of a hydrogen atom, oxygen is reduced to water (H 2 O) or hydrogen peroxide (H 2 O 2 ).
Pseudomonas oryzihabitans is a nonfermenting yellow-pigmented, gram-negative, rod-shaped bacterium that can cause sepsis, peritonitis, endophthalmitis, and bacteremia. [1] It is an opportunistic pathogen of humans and warm-blooded animals that is commonly found in several environmental sources, from soil to rice paddies.
Acinetobacter is a genus of Gram-negative bacteria belonging to the wider class of Gammaproteobacteria. Acinetobacter species are oxidase-negative, exhibit twitching motility, [7] and occur in pairs under magnification. They are important soil organisms, where they contribute to the mineralization of, for example, aromatic compounds.
Pseudomonas species also typically give a positive result to the oxidase test, the absence of gas formation from glucose, glucose is oxidised in oxidation/fermentation test using Hugh and Leifson O/F test, beta hemolytic (on blood agar), indole negative, methyl red negative, Voges–Proskauer test negative, and citrate positive. [citation needed]
Vibrio metschnikovii is a halophilic Gram-negative rod. They are facultatively anaerobic and motile through a single polar flagellum. [4] They are catalase positive. V. metschnikovii is the only pathogenic Vibrio that is oxidase negative. [5] They grow readily on routine laboratory including TCBS agar and blood agar.
Klebsiella is a genus of Gram-negative, oxidase-negative, rod-shaped bacteria with a prominent polysaccharide-based capsule. [3] Klebsiella is named after German-Swiss microbiologist Edwin Klebs (1834–1913). Carl Friedlander described Klebsiella bacillus which is why it was termed Friedlander bacillus for many years.
Klebsiella aerogenes, [2] previously known as Enterobacter aerogenes, is a Gram-negative, oxidase-negative, catalase-positive, citrate-positive, indole-negative, rod-shaped bacterium. [3] Capable of motility via peritrichous flagella, [4] it is approximately one to three microns in length.