Search results
Results from the WOW.Com Content Network
Hydroxyproline is found in few proteins other than collagen. For this reason, hydroxyproline content has been used as an indicator to determine collagen and/or gelatin amount. However, the mammalian proteins elastin and argonaute 2 have collagen-like domains in which hydroxyproline is formed.
Hyperprolinemia type II results in proline levels in the blood between 10 and 15 times higher than normal, and high levels of a related compound called pyrroline-5-carboxylate. This rare form of the disorder may appear benign at times, [ 2 ] but often involves seizures, convulsions, and intellectual disability.
In enzymology, a 4-hydroxyproline epimerase (EC 5.1.1.8) is an enzyme that catalyzes the chemical reaction trans -4-hydroxy-L-proline ⇌ {\displaystyle \rightleftharpoons } cis -4-hydroxy-D-proline Hence, this enzyme has one substrate , trans-4-hydroxy-L-proline , and one product , cis-4-hydroxy-D-proline .
Adams E; Goldstone A (1960). "Hydroxyproline metabolism. III. Enzymatic synthesis of hydroxyproline from Delta1-pyrroline-3-hydroxy-5-carboxylate".
Procollagen-proline dioxygenase catalyzes the following reaction: L-proline + alpha-ketoglutaric acid + O 2 → (2S, 4R)-4-hydroxyproline + succinate + CO 2. The mechanism for the reaction is similar to that of other dioxygenases, and occurs in two distinct stages: [3] In the first, a highly reactive Fe(IV)=O species is produced.
Three members of GT14 named GlcAT14A, GlcAT14B, and GlcAT14C were reported to add GlcA to both β-1,6- and β-1,3-Gal chains in an in vitro enzyme assay following heterologous expression in Pichia pastoris. [54] Two α-fucosyltransferase genes, FUT4 and FUT6, both belonging to GT37 family, encode enzymes which add α-1,2-fucose residues to AGPs.
As a result, the total concentration of protein in the sample can be deduced from the concentration of tryptophan and tyrosine residues that reduce the Folin–Ciocalteu reagent. The method was first proposed by Lowry in 1951. The bicinchoninic acid assay and the Hartree–Lowry assay are subsequent modifications of the original Lowry procedure.
The PFA-100 (Platelet Function Assay [1] or Platelet Function Analyser [2]) is a platelet function analyser that aspirates blood in vitro from a blood specimen into disposable test cartridges through a microscopic aperture cut into a biologically active membrane at the end of a capillary.