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The incubator maintains optimal temperature, humidity and other conditions such as the CO 2 and oxygen content of the atmosphere inside. Incubators are essential for much experimental work in cell biology , microbiology and molecular biology and are used to culture both bacterial and eukaryotic cells.
Geobacillus stearothermophilus (previously Bacillus stearothermophilus) [1] [2] is a rod-shaped, Gram-positive bacterium and a member of the phylum Bacillota.The bacterium is a thermophile and is widely distributed in soil, hot springs, ocean sediment, and is a cause of spoilage in food products.
The dilution plates are then incubated at a temperature of 37 degrees Celsius. [4] The plates are then incubated for sixteen to eighteen hours, although incubation time may be less for bacteria populations that divide quickly. [1]: 374 After incubation, the plates are examined to determine if bacterial growth has occurred in the inoculated ...
The phase-change incubator is a low-cost, low-maintenance incubator that tests for microorganisms in water supplies. It uses small balls containing a chemical compound that, when heated and then kept insulated, will stay at 37 °C (approx. 99 °F) for 24 hours.
Bacterial culture of H. influenzae is performed on agar plates. The strongest growth is seen on chocolate agar at 37 °C in a CO 2-enriched incubator. [33] The ideal CO 2 concentration for the culture is ~5%. [34] However adequate growth is often seen on brain-heart infusion agar supplemented with hemin and nicotinamide adenine dinucleotide (NAD)
The sealed anaerobic jar is then incubated at a desired temperature to allow growth of the bacteria. In the above figure for example, the incubation occurred at 100 °F (38 °C). A colorless indicator strip provides proof that the anaerobic conditions were met and the agar plates can now be observed for bacterial growth.
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The molten agar is cooled to about 45 degrees Celsius and is poured using a sterile method into a petri dish containing a specific diluted sample. From here, the plates are rotated to ensure the samples are uniformly mixing with the agar. Incubation of the plates is the next step and is carried out for about 3 days at 20 to 30 degrees Celsius.
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